238648
VIGCK6G3
2018
1
apa
50
date
asc
851
https://www.edyp.fr/web/wp-content/plugins/zotpress/
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Eymard-Vernain, E., Coute, Y., Adrait, A., Rabilloud, T., Sarret, G., & Lelong, C. (2018). The poly-gamma-glutamate of Bacillus subtilis interacts specifically with silver nanoparticles. PloS One, 13(5), e0197501. https://doi.org/10.1371/journal.pone.0197501
Govin, J., Barral, S., Morozumi, Y., Hoghoughi, N., Buchou, T., Rousseaux, S., & Khochbin, S. (2018). Characterization of Post-Meiotic Male Germ Cell Genome Organizational States. Methods in Molecular Biology (Clifton, N.J.), 1832, 293–307. https://doi.org/10.1007/978-1-4939-8663-7_16
Salvi, D., Bournais, S., Moyet, L., Bouchnak, I., Kuntz, M., Bruley, C., & Rolland, N. (2018). AT_CHLORO: The First Step When Looking for Information About Subplastidial Localization of Proteins. Methods in Molecular Biology (Clifton, N.J.), 1829, 395–406. https://doi.org/10.1007/978-1-4939-8654-5_26
El Kennani, S., Adrait, A., Permiakova, O., Hesse, A.-M., Ialy-Radio, C., Ferro, M., Brun, V., Cocquet, J., Govin, J., & Pflieger, D. (2018). Systematic quantitative analysis of H2A and H2B variants by targeted proteomics. Epigenetics & Chromatin, 11(1), 2. https://doi.org/10.1186/s13072-017-0172-y
De Muyt, A., Pyatnitskaya, A., Andréani, J., Ranjha, L., Ramus, C., Laureau, R., Fernandez-Vega, A., Holoch, D., Girard, E., Govin, J., Margueron, R., Couté, Y., Cejka, P., Guérois, R., & Borde, V. (2018). A meiotic XPF-ERCC1-like complex recognizes joint molecule recombination intermediates to promote crossover formation. Genes & Development, 32(3–4), 283–296. https://doi.org/10.1101/gad.308510.117
Adam, C., Guérois, R., Citarella, A., Verardi, L., Adolphe, F., Béneut, C., Sommermeyer, V., Ramus, C., Govin, J., Couté, Y., & Borde, V. (2018). The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes. PLoS Genetics, 14(2), e1007223. https://doi.org/10.1371/journal.pgen.1007223
Legendre, M., Fabre, E., Poirot, O., Jeudy, S., Lartigue, A., Alempic, J.-M., Beucher, L., Philippe, N., Bertaux, L., Christo-Foroux, E., Labadie, K., Couté, Y., Abergel, C., & Claverie, J.-M. (2018). Diversity and evolution of the emerging Pandoraviridae family. Nature Communications, 9(1), 2285. https://doi.org/10.1038/s41467-018-04698-4
Picard, M. A. L., Cosseau, C., Ferré, S., Quack, T., Grevelding, C. G., Couté, Y., & Vicoso, B. (2018). Evolution of gene dosage on the Z-chromosome of schistosome parasites. ELife, 7. https://doi.org/10.7554/eLife.35684
He, H., Brenier-Pinchart, M.-P., Braun, L., Kraut, A., Touquet, B., Couté, Y., Tardieux, I., Hakimi, M.-A., & Bougdour, A. (2018). Characterization of a Toxoplasma effector uncovers an alternative GSK3/β-catenin-regulatory pathway of inflammation. ELife, 7. https://doi.org/10.7554/eLife.39887
Rayapuram, N., Bigeard, J., Alhoraibi, H., Bonhomme, L., Hesse, A.-M., Vinh, J., Hirt, H., & Pflieger, D. (2018). Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets ofArabidopsisMitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6. Molecular & Cellular Proteomics: MCP, 17(1), 61–80. https://doi.org/10.1074/mcp.RA117.000135
Burger, T. (2018). Gentle Introduction to the Statistical Foundations of False Discovery Rate in Quantitative Proteomics. Journal of Proteome Research, 17(1), 12–22. https://doi.org/10.1021/acs.jproteome.7b00170
Petosa, C., Govin, J., & Mietton, F. (2018). [A new hope to fight invasive fungal infection]. Medecine Sciences: M/S, 34(2), 123–125. https://doi.org/10.1051/medsci/20183402007
Lacombe, M., Marie-Desvergne, C., Combes, F., Kraut, A., Bruley, C., Vandenbrouck, Y., Chamel Mossuz, V., Couté, Y., & Brun, V. (2018). Proteomic characterization of human exhaled breath condensate. Journal of Breath Research, 12(2), 021001. https://doi.org/10.1088/1752-7163/aa9e71
Garnaud, C., García-Oliver, E., Wang, Y., Maubon, D., Bailly, S., Despinasse, Q., Champleboux, M., Govin, J., & Cornet, M. (2018). The Rim Pathway Mediates Antifungal Tolerance in Candida albicans through Newly Identified Rim101 Transcriptional Targets, Including Hsp90 and Ipt1. Antimicrobial Agents and Chemotherapy, 62(3). https://doi.org/10.1128/AAC.01785-17
Abulfaraj, A. A., Mariappan, K., Bigeard, J., Manickam, P., Blilou, I., Guo, X., Al-Babili, S., Pflieger, D., Hirt, H., & Rayapuram, N. (2018). The Arabidopsis homolog of human G3BP1 is a key regulator of stomatal and apoplastic immunity. Life Science Alliance, 1(2), e201800046. https://doi.org/10.26508/lsa.201800046
Fröhlich, T., Hahn, F., Belmudes, L., Leidenberger, M., Friedrich, O., Kappes, B., Couté, Y., Marschall, M., & Tsogoeva, S. B. (2018). Synthesis of Artemisinin-Derived Dimers, Trimers and Dendrimers: Investigation of Their Antimalarial and Antiviral Activities Including Putative Mechanisms of Action. Chemistry (Weinheim an Der Bergstrasse, Germany), 24(32), 8103–8113. https://doi.org/10.1002/chem.201800729
Jacob, L., Combes, F., & Burger, T. (2018). PEPA test: fast and powerful differential analysis from relative quantitative proteomics data using shared peptides. Biostatistics (Oxford, England). https://doi.org/10.1093/biostatistics/kxy021
El Kennani, S., Crespo, M., Govin, J., & Pflieger, D. (2018). Proteomic Analysis of Histone Variants and Their PTMs: Strategies and Pitfalls. Proteomes, 6(3). https://doi.org/10.3390/proteomes6030029
Sage, E., Sansa, M., Fostner, S., Defoort, M., Gély, M., Naik, A. K., Morel, R., Duraffourg, L., Roukes, M. L., Alava, T., Jourdan, G., Colinet, E., Masselon, C., Brenac, A., & Hentz, S. (2018). Single-particle mass spectrometry with arrays of frequency-addressed nanomechanical resonators. Nature Communications, 9(1), 3283. https://doi.org/10.1038/s41467-018-05783-4
Mato, J. M., Elortza, F., Lu, S. C., Brun, V., Paradela, A., & Corrales, F. J. (2018). Liver cancer-associated changes to the proteome: what deserves clinical focus? Expert Review of Proteomics, 15(9), 749–756. https://doi.org/10.1080/14789450.2018.1521277
Nguyen, M. V. C., Adrait, A., Baillet, A., Trocmé, C., Gottenberg, J.-E., & Gaudin, P. (2018). Identification of cartilage oligomeric matrix protein as biomarker predicting abatacept response in rheumatoid arthritis patients with insufficient response to a first anti-TNFα treatment. Joint, Bone, Spine: Revue Du Rhumatisme. https://doi.org/10.1016/j.jbspin.2018.09.005
Shiota, H., Barral, S., Buchou, T., Tan, M., Couté, Y., Charbonnier, G., Reynoird, N., Boussouar, F., Gérard, M., Zhu, M., Bargier, L., Puthier, D., Chuffart, F., Bourova-Flin, E., Picaud, S., Filippakopoulos, P., Goudarzi, A., Ibrahim, Z., Panne, D., … Khochbin, S. (2018). Nut Directs p300-Dependent, Genome-Wide H4 Hyperacetylation in Male Germ Cells. Cell Reports, 24(13), 3477-3487.e6. https://doi.org/10.1016/j.celrep.2018.08.069
Völz, R., Kim, S.-K., Mi, J., Mariappan, K. G., Guo, X., Bigeard, J., Alejandro, S., Pflieger, D., Rayapuram, N., Al-Babili, S., & Hirt, H. (2018). The Trihelix transcription factor GT2-like 1 (GTL1) promotes salicylic acid metabolism, and regulates bacterial-triggered immunity. PLoS Genetics, 14(10), e1007708. https://doi.org/10.1371/journal.pgen.1007708
Hahn, F., Hutterer, C., Henry, C., Hamilton, S. T., Strojan, H., Kraut, A., Schulte, U., Schütz, M., Kohrt, S., Wangen, C., Pfizer, J., Couté, Y., Rawlinson, W. D., Strobl, S., & Marschall, M. (2018). Novel cytomegalovirus-inhibitory compounds of the class pyrrolopyridines show a complex pattern of target binding that suggests an unusual mechanism of antiviral activity. Antiviral Research, 159, 84–94. https://doi.org/10.1016/j.antiviral.2018.09.012
Albaret, M. A., Vermot-Desroches, C., Paré, A., Roca-Martinez, J.-X., Malet, L., Esseily, J., Gerossier, L., Brière, J., Pion, N., Marcel, V., Catez, F., De Souza, G., Vuillermoz, B., Doerflinger, F., Lavocat, E., Subiger, O., Rousset, C., Bresson, C., Mandon, E., … Diaz, J.-J. (2018). Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells. Cancers, 10(11). https://doi.org/10.3390/cancers10110452
Dominguez-Medina, S., Fostner, S., Defoort, M., Sansa, M., Stark, A.-K., Halim, M. A., Vernhes, E., Gely, M., Jourdan, G., Alava, T., Boulanger, P., Masselon, C., & Hentz, S. (2018). Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators. Science (New York, N.Y.), 362(6417), 918–922. https://doi.org/10.1126/science.aat6457
Jonckheere, J., Deloulme, J.-C., Dall’Igna, G., Chauliac, N., Pelluet, A., Nguon, A.-S., Lentini, C., Brocard, J., Denarier, E., Brugière, S., Couté, Y., Heinrich, C., Porcher, C., Holtzmann, J., Andrieux, A., Suaud-Chagny, M.-F., & Gory-Fauré, S. (2018). Short- and long-term efficacy of electroconvulsive stimulation in animal models of depression: The essential role of neuronal survival. Brain Stimulation, 11(6), 1336–1347. https://doi.org/10.1016/j.brs.2018.08.001