238648
VIGCK6G3
2021
1
apa
50
date
asc
1071
https://www.edyp.fr/web/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-ac0712ae3da8e40515ebc010818996fc%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22VAVRCVKZ%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Takata-Tsuji%20et%20al.%22%2C%22parsedDate%22%3A%222021-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ETakata-Tsuji%2C%20F.%2C%20Chounlamountri%2C%20N.%2C%20Do%2C%20L.-D.%2C%20Philippot%2C%20C.%2C%20Novion%20Ducassou%2C%20J.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Ben%20Achour%2C%20S.%2C%20Honnorat%2C%20J.%2C%20Place%2C%20C.%2C%20%26amp%3B%20Pascual%2C%20O.%20%282021%29.%20Microglia%20modulate%20gliotransmission%20through%20the%20regulation%20of%20VAMP2%20proteins%20in%20astrocytes.%20%3Ci%3EGlia%3C%5C%2Fi%3E%2C%20%3Ci%3E69%3C%5C%2Fi%3E%281%29%2C%2061%26%23x2013%3B72.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fglia.23884%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fglia.23884%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Microglia%20modulate%20gliotransmission%20through%20the%20regulation%20of%20VAMP2%20proteins%20in%20astrocytes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fuyuko%22%2C%22lastName%22%3A%22Takata-Tsuji%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Naura%22%2C%22lastName%22%3A%22Chounlamountri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Le-Duy%22%2C%22lastName%22%3A%22Do%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camille%22%2C%22lastName%22%3A%22Philippot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%22%2C%22lastName%22%3A%22Novion%20Ducassou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sarrah%22%2C%22lastName%22%3A%22Ben%20Achour%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J%5Cu00e9r%5Cu00f4me%22%2C%22lastName%22%3A%22Honnorat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Place%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Pascual%22%7D%5D%2C%22abstractNote%22%3A%22Vesicular%20release%20is%20one%20of%20the%20release%20mechanisms%20of%20various%20signaling%20molecules.%20In%20neurons%2C%20the%20molecular%20machinery%20involved%20in%20vesicular%20release%20has%20been%20designed%20through%20evolution%20to%20trigger%20fast%20and%20synchronous%20release%20of%20neurotransmitters.%20Similar%20machinery%20with%20a%20slower%20kinetic%20and%20a%20slightly%20different%20molecular%20assembly%20allows%20astrocytes%20to%20release%20various%20transmitters%20such%20as%20adenosine%20triphosphate%20%28ATP%29%2C%20glutamate%2C%20and%20D-serine.%20Astrocytes%20are%20important%20modulators%20of%20neurotransmission%20through%20gliotransmitter%20release.%20We%20recently%20demonstrated%20that%20microglia%2C%20another%20type%20of%20glia%2C%20release%20ATP%20to%20modulate%20synaptic%20transmission%20using%20astrocytes%20as%20intermediate.%20We%20now%20report%20that%20microglia%20regulate%20astrocytic%20gliotransmission%20through%20the%20regulation%20of%20SNARE%20proteins%20in%20astrocytes.%20Indeed%2C%20we%20found%20that%20gliotransmission%20triggered%20by%20P2Y1%20agonist%20is%20impaired%20in%20slices%20from%20transgenic%20mice%20devoid%20of%20microglia.%20Using%20total%20internal%20reflection%20fluorescence%20imaging%2C%20we%20found%20that%20the%20vesicular%20release%20of%20gliotransmitter%20by%20astrocytes%20was%20different%20in%20cultures%20lacking%20microglia%20compared%20to%20vesicular%20release%20in%20astrocytes%20cocultured%20with%20microglia.%20Quantification%20of%20the%20kinetic%20of%20vesicular%20release%20indicates%20that%20the%20overall%20release%20appears%20to%20be%20faster%20in%20pure%20astrocyte%20cultures%20with%20more%20vesicles%20close%20to%20the%20membrane%20when%20compared%20to%20astrocytes%20cocultured%20with%20microglia.%20Finally%2C%20biochemical%20investigation%20of%20SNARE%20protein%20expression%20indicates%20an%20upregulation%20of%20VAMP2%20in%20absence%20of%20microglia.%20Altogether%2C%20these%20results%20indicate%20that%20microglia%20seems%20to%20be%20involved%20in%20the%20regulation%20of%20an%20astrocytic%20phenotype%20compatible%20with%20proper%20gliotransmission.%20The%20mechanisms%20described%20in%20this%20study%20could%20be%20of%20importance%20for%20central%20nervous%20system%20diseases%20where%20microglia%20are%20activated.%22%2C%22date%22%3A%222021-01%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1002%5C%2Fglia.23884%22%2C%22ISSN%22%3A%221098-1136%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A09Z%22%7D%7D%2C%7B%22key%22%3A%22S2YJUVIS%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Adrait%20et%20al.%22%2C%22parsedDate%22%3A%222021-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAdrait%2C%20A.%2C%20Dumonceau%2C%20J.%2C%20Delhaye%2C%20M.%2C%20Annessi%26%23x2010%3BRamseyer%2C%20I.%2C%20Frossard%2C%20J.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20%26amp%3B%20Farina%2C%20A.%20%282021%29.%20Liquid%20Biopsy%20of%20Bile%20based%20on%20Targeted%20Mass%20Spectrometry%20for%20the%20Diagnosis%20of%20Malignant%20Biliary%20Strictures.%20%3Ci%3EClinical%20and%20Translational%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3E14%3C%5C%2Fi%3E%281%29%2C%20148%26%23x2013%3B152.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fcts.12890%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fcts.12890%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Liquid%20Biopsy%20of%20Bile%20based%20on%20Targeted%20Mass%20Spectrometry%20for%20the%20Diagnosis%20of%20Malignant%20Biliary%20Strictures%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annie%22%2C%22lastName%22%3A%22Adrait%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean%5Cu2010Marc%22%2C%22lastName%22%3A%22Dumonceau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Delhaye%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isabelle%22%2C%22lastName%22%3A%22Annessi%5Cu2010Ramseyer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean%5Cu2010Louis%22%2C%22lastName%22%3A%22Frossard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annarita%22%2C%22lastName%22%3A%22Farina%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1111%5C%2Fcts.12890%22%2C%22ISSN%22%3A%221752-8054%2C%201752-8062%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2F10.1111%5C%2Fcts.12890%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A10Z%22%7D%7D%2C%7B%22key%22%3A%223E9S2HWQ%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Felix%20et%20al.%22%2C%22parsedDate%22%3A%222021-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFelix%2C%20J.%2C%20Siebert%2C%20C.%2C%20Ducassou%2C%20J.%20N.%2C%20Nigou%2C%20J.%2C%20Garcia%2C%20P.%20S.%2C%20Fraudeau%2C%20A.%2C%20Huard%2C%20K.%2C%20Mas%2C%20C.%2C%20Brochier-Armanet%2C%20C.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Gutsche%2C%20I.%2C%20%26amp%3B%20Renesto%2C%20P.%20%282021%29.%20Structural%20and%20functional%20analysis%20of%20the%20Francisella%20lysine%20decarboxylase%20as%20a%20key%20actor%20in%20oxidative%20stress%20resistance.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%20972.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-020-79611-5%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-020-79611-5%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Structural%20and%20functional%20analysis%20of%20the%20Francisella%20lysine%20decarboxylase%20as%20a%20key%20actor%20in%20oxidative%20stress%20resistance%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jan%22%2C%22lastName%22%3A%22Felix%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22Siebert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%20Novion%22%2C%22lastName%22%3A%22Ducassou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J%5Cu00e9r%5Cu00f4me%22%2C%22lastName%22%3A%22Nigou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%20Simon%22%2C%22lastName%22%3A%22Garcia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ang%5Cu00e9lique%22%2C%22lastName%22%3A%22Fraudeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karine%22%2C%22lastName%22%3A%22Huard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Mas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9line%22%2C%22lastName%22%3A%22Brochier-Armanet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Irina%22%2C%22lastName%22%3A%22Gutsche%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricia%22%2C%22lastName%22%3A%22Renesto%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Francisella%20tularensis%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20is%20one%20of%20the%20most%20virulent%20pathogenic%20bacteria%20causing%20the%20acute%20human%20respiratory%20disease%20tularemia.%20While%20the%20mechanisms%20underlying%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20F.%20tularensis%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20pathogenesis%20are%20largely%20unknown%2C%20previous%20studies%20have%20shown%20that%20a%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20F.%20novicida%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20transposon%20mutant%20with%20insertions%20in%20a%20gene%20coding%20for%20a%20putative%20lysine%20decarboxylase%20was%20attenuated%20in%20mouse%20spleen%2C%20suggesting%20a%20possible%20role%20of%20its%20protein%20product%20as%20a%20virulence%20factor.%20Therefore%2C%20we%20set%20out%20to%20structurally%20and%20functionally%20characterize%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20F.%20novicida%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20lysine%20decarboxylase%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20which%20we%20termed%20LdcF%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Here%2C%20we%20investigate%20the%20genetic%20environment%20of%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20ldcF%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20as%20well%20as%20its%20evolutionary%20relationships%20with%20other%20basic%20AAT-fold%20amino%20acid%20decarboxylase%20superfamily%20members%2C%20known%20as%20key%20actors%20in%20bacterial%20adaptative%20stress%20response%20and%20polyamine%20biosynthesis.%20We%20determine%20the%20crystal%20structure%20of%20LdcF%20and%20compare%20it%20with%20the%20most%20thoroughly%20studied%20lysine%20decarboxylase%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20E.%20coli%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20LdcI%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20We%20analyze%20the%20influence%20of%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20ldcF%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20deletion%20on%20bacterial%20growth%20under%20different%20stress%20conditions%20in%20dedicated%20growth%20media%2C%20as%20well%20as%20in%20infected%20macrophages%2C%20and%20demonstrate%20its%20involvement%20in%20oxidative%20stress%20resistance.%20Finally%2C%20our%20mass%20spectrometry-based%20quantitative%20proteomic%20analysis%20enables%20identification%20of%2080%20proteins%20with%20expression%20levels%20significantly%20affected%20by%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20ldcF%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20deletion%2C%20including%20several%20DNA%20repair%20proteins%20potentially%20involved%20in%20the%20diminished%20capacity%20of%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20F.%20novicida%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20mutant%20to%20deal%20with%20oxidative%20stress.%20Taken%20together%2C%20we%20uncover%20an%20important%20role%20of%20LdcF%20in%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20F.%20novicida%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20survival%20in%20host%20cells%20through%20participation%20in%20oxidative%20stress%20response%2C%20thereby%20singling%20out%20this%20previously%20uncharacterized%20protein%20as%20a%20potential%20drug%20target.%22%2C%22date%22%3A%222021-01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-020-79611-5%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-020-79611-5%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A10Z%22%7D%7D%2C%7B%22key%22%3A%229KV6ABI5%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Kerjouan%20et%20al.%22%2C%22parsedDate%22%3A%222021-01-15%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKerjouan%2C%20A.%2C%20Boyault%2C%20C.%2C%20Oddou%2C%20C.%2C%20Hiriart-Bryant%2C%20E.%2C%20Grichine%2C%20A.%2C%20Kraut%2C%20A.%2C%20Pezet%2C%20M.%2C%20Balland%2C%20M.%2C%20Faurobert%2C%20E.%2C%20Bonnet%2C%20I.%2C%20Coute%2C%20Y.%2C%20Fourcade%2C%20B.%2C%20Albiges-Rizo%2C%20C.%2C%20%26amp%3B%20Destaing%2C%20O.%20%282021%29.%20Control%20of%20SRC%20molecular%20dynamics%20encodes%20distinct%20cytoskeletal%20responses%20by%20specifying%20signaling%20pathway%20usage.%20%3Ci%3EJournal%20of%20Cell%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3E134%3C%5C%2Fi%3E%282%29%2C%20jcs254599.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1242%5C%2Fjcs.254599%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1242%5C%2Fjcs.254599%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Control%20of%20SRC%20molecular%20dynamics%20encodes%20distinct%20cytoskeletal%20responses%20by%20specifying%20signaling%20pathway%20usage%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ad%5Cu00e8le%22%2C%22lastName%22%3A%22Kerjouan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cyril%22%2C%22lastName%22%3A%22Boyault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christiane%22%2C%22lastName%22%3A%22Oddou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Edwige%22%2C%22lastName%22%3A%22Hiriart-Bryant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexei%22%2C%22lastName%22%3A%22Grichine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Kraut%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myl%5Cu00e8ne%22%2C%22lastName%22%3A%22Pezet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martial%22%2C%22lastName%22%3A%22Balland%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eva%22%2C%22lastName%22%3A%22Faurobert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Isabelle%22%2C%22lastName%22%3A%22Bonnet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Coute%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bertrand%22%2C%22lastName%22%3A%22Fourcade%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Corinne%22%2C%22lastName%22%3A%22Albiges-Rizo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Destaing%22%7D%5D%2C%22abstractNote%22%3A%22ABSTRACT%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Upon%20activation%20by%20different%20transmembrane%20receptors%2C%20the%20same%20signaling%20protein%20can%20induce%20distinct%20cellular%20responses.%20A%20way%20to%20decipher%20the%20mechanisms%20of%20such%20pleiotropic%20signaling%20activity%20is%20to%20directly%20manipulate%20the%20decision-making%20activity%20that%20supports%20the%20selection%20between%20distinct%20cellular%20responses.%20We%20developed%20an%20optogenetic%20probe%20%28optoSRC%29%20to%20control%20SRC%20signaling%2C%20an%20example%20of%20a%20pleiotropic%20signaling%20node%2C%20and%20we%20demonstrated%20its%20ability%20to%20generate%20different%20acto-adhesive%20structures%20%28lamellipodia%20or%20invadosomes%29%20upon%20distinct%20spatio-temporal%20control%20of%20SRC%20kinase%20activity.%20The%20occurrence%20of%20each%20acto-adhesive%20structure%20was%20simply%20dictated%20by%20the%20dynamics%20of%20optoSRC%20nanoclusters%20in%20adhesive%20sites%2C%20which%20were%20dependent%20on%20the%20SH3%20and%20Unique%20domains%20of%20the%20protein.%20The%20different%20decision-making%20events%20regulated%20by%20optoSRC%20dynamics%20induced%20distinct%20downstream%20signaling%20pathways%2C%20which%20we%20characterized%20using%20time-resolved%20proteomic%20and%20network%20analyses.%20Collectively%2C%20by%20manipulating%20the%20molecular%20mobility%20of%20SRC%20kinase%20activity%2C%20these%20experiments%20reveal%20the%20pleiotropy-encoding%20mechanism%20of%20SRC%20signaling.%22%2C%22date%22%3A%222021-01-15%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1242%5C%2Fjcs.254599%22%2C%22ISSN%22%3A%220021-9533%2C%201477-9137%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.biologists.com%5C%2Fjcs%5C%2Farticle%5C%2F134%5C%2F2%5C%2Fjcs254599%5C%2F237349%5C%2FControl-of-SRC-molecular-dynamics-encodes-distinct%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A09Z%22%7D%7D%2C%7B%22key%22%3A%22ZXCKMG69%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Gilquin%20et%20al.%22%2C%22parsedDate%22%3A%222021-01-19%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGilquin%2C%20B.%2C%20Cubizolles%2C%20M.%2C%20Den%20Dulk%2C%20R.%2C%20Revol-Cavalier%2C%20F.%2C%20Alessio%2C%20M.%2C%20Goujon%2C%20C.-E.%2C%20Echampard%2C%20C.%2C%20Arrizabalaga%2C%20G.%2C%20Adrait%2C%20A.%2C%20Louwagie%2C%20M.%2C%20Laurent%2C%20P.%2C%20Navarro%2C%20F.%20P.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Cosnier%2C%20M.-L.%2C%20%26amp%3B%20Brun%2C%20V.%20%282021%29.%20PepS%3A%20An%20Innovative%20Microfluidic%20Device%20for%20Bedside%20Whole%20Blood%20Processing%20before%20Plasma%20Proteomics%20Analyses.%20%3Ci%3EAnalytical%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E93%3C%5C%2Fi%3E%282%29%2C%20683%26%23x2013%3B690.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.analchem.0c02270%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.analchem.0c02270%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22PepS%3A%20An%20Innovative%20Microfluidic%20Device%20for%20Bedside%20Whole%20Blood%20Processing%20before%20Plasma%20Proteomics%20Analyses%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoit%22%2C%22lastName%22%3A%22Gilquin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Cubizolles%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Remco%22%2C%22lastName%22%3A%22Den%20Dulk%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fr%5Cu00e9d%5Cu00e9ric%22%2C%22lastName%22%3A%22Revol-Cavalier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manuel%22%2C%22lastName%22%3A%22Alessio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charles-Elie%22%2C%22lastName%22%3A%22Goujon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Camille%22%2C%22lastName%22%3A%22Echampard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gorka%22%2C%22lastName%22%3A%22Arrizabalaga%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annie%22%2C%22lastName%22%3A%22Adrait%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathilde%22%2C%22lastName%22%3A%22Louwagie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricia%22%2C%22lastName%22%3A%22Laurent%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fabrice%20P.%22%2C%22lastName%22%3A%22Navarro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Line%22%2C%22lastName%22%3A%22Cosnier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Virginie%22%2C%22lastName%22%3A%22Brun%22%7D%5D%2C%22abstractNote%22%3A%22Immunoassays%20have%20been%20used%20for%20decades%20in%20clinical%20laboratories%20to%20quantify%20proteins%20in%20serum%20and%20plasma%20samples.%20However%2C%20their%20limitations%20make%20them%20inappropriate%20in%20some%20cases.%20Recently%2C%20mass%20spectrometry%20%28MS%29%20based%20proteomics%20analysis%20has%20emerged%20as%20a%20promising%20alternative%20method%20when%20seeking%20to%20assess%20panels%20of%20protein%20biomarkers%20with%20a%20view%20to%20providing%20protein%20profiles%20to%20monitor%20health%20status.%20Up%20to%20now%2C%20however%2C%20translation%20of%20MS-based%20proteomics%20to%20the%20clinic%20has%20been%20hampered%20by%20its%20complexity%20and%20the%20substantial%20time%20and%20human%20resources%20necessary%20for%20sample%20preparation.%20Plasma%20matrix%20is%20particularly%20tricky%20to%20process%20as%20it%20contains%20more%20than%203000%20proteins%20with%20concentrations%20spanning%20an%20extreme%20dynamic%20range%20%281010%29.%20To%20address%20this%20preanalytical%20challenge%2C%20we%20designed%20a%20microfluidic%20device%20%28PepS%29%20automating%20and%20accelerating%20blood%20sample%20preparation%20for%20bottom-up%20MS-based%20proteomics%20analysis.%20The%20microfluidic%20cartridge%20is%20operated%20through%20a%20dedicated%20compact%20instrument%20providing%20fully%20automated%20fluid%20processing%20and%20thermal%20control.%20In%20less%20than%202%20h%2C%20the%20PepS%20device%20allows%20bedside%20plasma%20separation%20from%20whole%20blood%2C%20volume%20metering%2C%20depletion%20of%20albumin%2C%20protein%20digestion%20with%20trypsin%2C%20and%20stabilization%20of%20tryptic%20peptides%20on%20solid-phase%20extraction%20sorbent.%20For%20this%20first%20presentation%2C%20the%20performance%20of%20the%20PepS%20device%20was%20assessed%20using%20discovery%20proteomics%20and%20targeted%20proteomics%2C%20detecting%20a%20panel%20of%20three%20protein%20biomarkers%20routinely%20assayed%20in%20clinical%20laboratories%20%28alanine%20aminotransferase%201%2C%20C-reactive%20protein%2C%20and%20myoglobin%29.%20This%20innovative%20microfluidic%20device%20and%20its%20associated%20instrumentation%20should%20help%20to%20streamline%20and%20simplify%20clinical%20proteomics%20studies.%22%2C%22date%22%3A%222021-01-19%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1021%5C%2Facs.analchem.0c02270%22%2C%22ISSN%22%3A%221520-6882%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A09Z%22%7D%7D%2C%7B%22key%22%3A%224GUWVEHU%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Rayapuram%20et%20al.%22%2C%22parsedDate%22%3A%222021-01-19%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERayapuram%2C%20N.%2C%20Jarad%2C%20M.%2C%20Alhoraibi%2C%20H.%20M.%2C%20Bigeard%2C%20J.%2C%20Abulfaraj%2C%20A.%20A.%2C%20V%26%23xF6%3Blz%2C%20R.%2C%20Mariappan%2C%20K.%20G.%2C%20Almeida-Trapp%2C%20M.%2C%20Schl%26%23xF6%3Bffel%2C%20M.%2C%20Lastrucci%2C%20E.%2C%20Bonhomme%2C%20L.%2C%20Gust%2C%20A.%20A.%2C%20Mith%26%23xF6%3Bfer%2C%20A.%2C%20Arold%2C%20S.%20T.%2C%20Pflieger%2C%20D.%2C%20%26amp%3B%20Hirt%2C%20H.%20%282021%29.%20Chromatin%20phosphoproteomics%20unravels%20a%20function%20for%20AT-hook%20motif%20nuclear%20localized%20protein%20AHL13%20in%20PAMP-triggered%20immunity.%20%3Ci%3EProceedings%20of%20the%20National%20Academy%20of%20Sciences%3C%5C%2Fi%3E%2C%20%3Ci%3E118%3C%5C%2Fi%3E%283%29%2C%20e2004670118.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2004670118%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1073%5C%2Fpnas.2004670118%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Chromatin%20phosphoproteomics%20unravels%20a%20function%20for%20AT-hook%20motif%20nuclear%20localized%20protein%20AHL13%20in%20PAMP-triggered%20immunity%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Naganand%22%2C%22lastName%22%3A%22Rayapuram%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mai%22%2C%22lastName%22%3A%22Jarad%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hanna%20M.%22%2C%22lastName%22%3A%22Alhoraibi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean%22%2C%22lastName%22%3A%22Bigeard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aala%20A.%22%2C%22lastName%22%3A%22Abulfaraj%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ronny%22%2C%22lastName%22%3A%22V%5Cu00f6lz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kiruthiga%20Gayathri%22%2C%22lastName%22%3A%22Mariappan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marilia%22%2C%22lastName%22%3A%22Almeida-Trapp%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Schl%5Cu00f6ffel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Lastrucci%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ludovic%22%2C%22lastName%22%3A%22Bonhomme%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrea%20A.%22%2C%22lastName%22%3A%22Gust%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Axel%22%2C%22lastName%22%3A%22Mith%5Cu00f6fer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stefan%20T.%22%2C%22lastName%22%3A%22Arold%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Delphine%22%2C%22lastName%22%3A%22Pflieger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Heribert%22%2C%22lastName%22%3A%22Hirt%22%7D%5D%2C%22abstractNote%22%3A%22Significance%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Mitogen-activated%20protein%20kinases%20%28MAPKs%29%20function%20in%20all%20eukaryotes%20in%20signaling%20extracellular%20stimuli%20to%20intracellular%20responses%20and%20ultimately%20link%20them%20to%20chromatin%20events%20by%20targeting%20transcription%20factors%20and%20chromatin%20remodeling%20complexes.%20In%20plants%2C%20MAPKs%20play%20crucial%20roles%20in%20immunity%2C%20development%2C%20and%20stress%20responses%2C%20but%20so%20far%20no%20attempts%20have%20been%20made%20to%20identify%20phosphorylation%20of%20chromatin-associated%20proteins.%20By%20using%20a%20phosphoproteomic%20approach%20on%20MAPK%20mutants%2C%20we%20identified%20a%20number%20of%20chromatin-associated%20MAPK%20substrates%20and%20characterize%20an%20AT-hook%20motif%20containing%20nuclear%20localized%20%28AHL%29%20DNA-binding%20protein%2013%20in%20plant%20immunity%20and%20demonstrate%20that%20phosphorylation%20regulates%20AHL13%20protein%20stability%20and%2C%20in%20turn%2C%20its%20function%20in%20response%20to%20pathogens.%20%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20In%20many%20eukaryotic%20systems%20during%20immune%20responses%2C%20mitogen-activated%20protein%20kinases%20%28MAPKs%29%20link%20cytoplasmic%20signaling%20to%20chromatin%20events%20by%20targeting%20transcription%20factors%2C%20chromatin%20remodeling%20complexes%2C%20and%20the%20RNA%20polymerase%20machinery.%20So%20far%2C%20knowledge%20on%20these%20events%20is%20scarce%20in%20plants%20and%20no%20attempts%20have%20been%20made%20to%20focus%20on%20phosphorylation%20events%20of%20chromatin-associated%20proteins.%20Here%20we%20carried%20out%20chromatin%20phosphoproteomics%20upon%20elicitor-induced%20activation%20of%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Arabidopsis%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20The%20events%20in%20WT%20were%20compared%20with%20those%20in%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20mpk3%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20mpk4%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20and%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20mpk6%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20mutant%20plants%20to%20decipher%20specific%20MAPK%20targets.%20Our%20study%20highlights%20distinct%20signaling%20networks%20involving%20MPK3%2C%20MPK4%2C%20and%20MPK6%20in%20chromatin%20organization%20and%20modification%2C%20as%20well%20as%20in%20RNA%20transcription%20and%20processing.%20Among%20the%20chromatin%20targets%2C%20we%20characterized%20the%20AT-hook%20motif%20containing%20nuclear%20localized%20%28AHL%29%20DNA-binding%20protein%20AHL13%20as%20a%20substrate%20of%20immune%20MAPKs.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20AHL13%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20knockout%20mutant%20plants%20are%20compromised%20in%20pathogen-associated%20molecular%20pattern%20%28PAMP%29-induced%20reactive%20oxygen%20species%20production%2C%20expression%20of%20defense%20genes%2C%20and%20PAMP-triggered%20immunity.%20Transcriptome%20analysis%20revealed%20that%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20AHL13%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20regulates%20key%20factors%20of%20jasmonic%20acid%20biosynthesis%20and%20signaling%20and%20affects%20immunity%20toward%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Pseudomonas%20syringae%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Botrytis%20cinerea%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20pathogens.%20Mutational%20analysis%20of%20the%20phosphorylation%20sites%20of%20AHL13%20demonstrated%20that%20phosphorylation%20regulates%20AHL13%20protein%20stability%20and%20thereby%20its%20immune%20functions.%22%2C%22date%22%3A%222021-01-19%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1073%5C%2Fpnas.2004670118%22%2C%22ISSN%22%3A%220027-8424%2C%201091-6490%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fpnas.org%5C%2Fdoi%5C%2Ffull%5C%2F10.1073%5C%2Fpnas.2004670118%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A08Z%22%7D%7D%2C%7B%22key%22%3A%22J7UHDJAZ%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Permiakova%20et%20al.%22%2C%22parsedDate%22%3A%222021-02%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPermiakova%2C%20O.%2C%20Guibert%2C%20R.%2C%20Kraut%2C%20A.%2C%20Fortin%2C%20T.%2C%20Hesse%2C%20A.-M.%2C%20%26amp%3B%20Burger%2C%20T.%20%282021%29.%20CHICKN%3A%20extraction%20of%20peptide%20chromatographic%20elution%20profiles%20from%20large%20scale%20mass%20spectrometry%20data%20by%20means%20of%20Wasserstein%20compressive%20hierarchical%20cluster%20analysis.%20%3Ci%3EBMC%20Bioinformatics%3C%5C%2Fi%3E%2C%20%3Ci%3E22%3C%5C%2Fi%3E%281%29%2C%2068.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12859-021-03969-0%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs12859-021-03969-0%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22CHICKN%3A%20extraction%20of%20peptide%20chromatographic%20elution%20profiles%20from%20large%20scale%20mass%20spectrometry%20data%20by%20means%20of%20Wasserstein%20compressive%20hierarchical%20cluster%20analysis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olga%22%2C%22lastName%22%3A%22Permiakova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Romain%22%2C%22lastName%22%3A%22Guibert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Kraut%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Fortin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne-Marie%22%2C%22lastName%22%3A%22Hesse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Burger%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Background%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20The%20clustering%20of%20data%20produced%20by%20liquid%20chromatography%20coupled%20to%20mass%20spectrometry%20analyses%20%28LC-MS%20data%29%20has%20recently%20gained%20interest%20to%20extract%20meaningful%20chemical%20or%20biological%20patterns.%20However%2C%20recent%20instrumental%20pipelines%20deliver%20data%20which%20size%2C%20dimensionality%20and%20expected%20number%20of%20clusters%20are%20too%20large%20to%20be%20processed%20by%20classical%20machine%20learning%20algorithms%2C%20so%20that%20most%20of%20the%20state-of-the-art%20relies%20on%20single%20pass%20linkage-based%20algorithms.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Results%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20We%20propose%20a%20clustering%20algorithm%20that%20solves%20the%20powerful%20but%20computationally%20demanding%20kernel%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20k%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20-means%20objective%20function%20in%20a%20scalable%20way.%20As%20a%20result%2C%20it%20can%20process%20LC-MS%20data%20in%20an%20acceptable%20time%20on%20a%20multicore%20machine.%20To%20do%20so%2C%20we%20combine%20three%20essential%20features%3A%20a%20compressive%20data%20representation%2C%20Nystr%5Cu00f6m%20approximation%20and%20a%20hierarchical%20strategy.%20In%20addition%2C%20we%20propose%20new%20kernels%20based%20on%20optimal%20transport%2C%20which%20interprets%20as%20intuitive%20similarity%20measures%20between%20chromatographic%20elution%20profiles.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Conclusions%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Our%20method%2C%20referred%20to%20as%20CHICKN%2C%20is%20evaluated%20on%20proteomics%20data%20produced%20in%20our%20lab%2C%20as%20well%20as%20on%20benchmark%20data%20coming%20from%20the%20literature.%20From%20a%20computational%20viewpoint%2C%20it%20is%20particularly%20efficient%20on%20raw%20LC-MS%20data.%20From%20a%20data%20analysis%20viewpoint%2C%20it%20provides%20clusters%20which%20differ%20from%20those%20resulting%20from%20state-of-the-art%20methods%2C%20while%20achieving%20similar%20performances.%20This%20highlights%20the%20complementarity%20of%20differently%20principle%20algorithms%20to%20extract%20the%20best%20from%20complex%20LC-MS%20data.%22%2C%22date%22%3A%222021-02%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1186%5C%2Fs12859-021-03969-0%22%2C%22ISSN%22%3A%221471-2105%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fbmcbioinformatics.biomedcentral.com%5C%2Farticles%5C%2F10.1186%5C%2Fs12859-021-03969-0%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A08Z%22%7D%7D%2C%7B%22key%22%3A%22UVHBKG6C%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Vellino%20et%20al.%22%2C%22parsedDate%22%3A%222021-02-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EVellino%2C%20S.%2C%20Oddou%2C%20C.%2C%20Rivier%2C%20P.%2C%20Boyault%2C%20C.%2C%20Hiriart-Bryant%2C%20E.%2C%20Kraut%2C%20A.%2C%20Martin%2C%20R.%2C%20Coute%2C%20Y.%2C%20Kn%26%23xF6%3Blker%2C%20H.-J.%2C%20Valverde%2C%20M.%20A.%2C%20Albig%26%23xE8%3Bs-Rizo%2C%20C.%2C%20%26amp%3B%20Destaing%2C%20O.%20%282021%29.%20Cross-talk%20between%20the%20calcium%20channel%20TRPV4%20and%20reactive%20oxygen%20species%20interlocks%20adhesive%20and%20degradative%20functions%20of%20invadosomes.%20%3Ci%3EJournal%20of%20Cell%20Biology%3C%5C%2Fi%3E%2C%20%3Ci%3E220%3C%5C%2Fi%3E%282%29%2C%20e201910079.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1083%5C%2Fjcb.201910079%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1083%5C%2Fjcb.201910079%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Cross-talk%20between%20the%20calcium%20channel%20TRPV4%20and%20reactive%20oxygen%20species%20interlocks%20adhesive%20and%20degradative%20functions%20of%20invadosomes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sanela%22%2C%22lastName%22%3A%22Vellino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christiane%22%2C%22lastName%22%3A%22Oddou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%22%2C%22lastName%22%3A%22Rivier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cyril%22%2C%22lastName%22%3A%22Boyault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Edwige%22%2C%22lastName%22%3A%22Hiriart-Bryant%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Kraut%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ren%5Cu00e9%22%2C%22lastName%22%3A%22Martin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Coute%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hans-Joachim%22%2C%22lastName%22%3A%22Kn%5Cu00f6lker%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miguel%20A.%22%2C%22lastName%22%3A%22Valverde%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Corinne%22%2C%22lastName%22%3A%22Albig%5Cu00e8s-Rizo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Destaing%22%7D%5D%2C%22abstractNote%22%3A%22Invadosomes%20support%20cell%20invasion%20by%20coupling%20both%20acto-adhesive%20and%20extracellular%20matrix%20degradative%20functions%2C%20which%20are%20apparently%20antagonistic.%20%5Cu03b21-integrin%20dynamics%20regulate%20this%20coupling%2C%20but%20the%20actual%20sensing%20mechanism%20and%20effectors%20involved%20have%20not%20yet%20been%20elucidated.%20Using%20genetic%20and%20reverse%20genetic%20approaches%20combined%20with%20biochemical%20and%20imaging%20techniques%2C%20we%20now%20show%20that%20the%20calcium%20channel%20TRPV4%20colocalizes%20with%20%5Cu03b21-integrins%20at%20the%20invadosome%20periphery%20and%20regulates%20its%20activation%20and%20the%20coupling%20of%20acto-adhesive%20and%20degradative%20functions.%20TRPV4-mediated%20regulation%20of%20podosome%20function%20depends%20on%20its%20ability%20to%20sense%20reactive%20oxygen%20species%20%28ROS%29%20in%20invadosomes%5Cu2019%20microenvironment%20and%20involves%20activation%20of%20the%20ROS%5C%2Fcalcium-sensitive%20kinase%20Ask1%20and%20binding%20of%20the%20motor%20MYO1C.%20Furthermore%2C%20disease-associated%20TRPV4%20gain-of-function%20mutations%20that%20modulate%20ECM%20degradation%20are%20also%20implicated%20in%20the%20ROS%20response%2C%20which%20provides%20new%20perspectives%20in%20our%20understanding%20of%20the%20pathophysiology%20of%20TRPV4%20channelopathies.%22%2C%22date%22%3A%222021-02-01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1083%5C%2Fjcb.201910079%22%2C%22ISSN%22%3A%220021-9525%2C%201540-8140%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Frupress.org%5C%2Fjcb%5C%2Farticle%5C%2Fdoi%5C%2F10.1083%5C%2Fjcb.201910079%5C%2F211651%5C%2FCross-talk-between-the-calcium-channel-TRPV4-and%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A08Z%22%7D%7D%2C%7B%22key%22%3A%22K3N4SF8F%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22van%20Lis%20et%20al.%22%2C%22parsedDate%22%3A%222021-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3Evan%20Lis%2C%20R.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Brugi%26%23xE8%3Bre%2C%20S.%2C%20Tourasse%2C%20N.%20J.%2C%20Laurent%2C%20B.%2C%20Nitschke%2C%20W.%2C%20Vallon%2C%20O.%2C%20%26amp%3B%20Atteia%2C%20A.%20%282021%29.%20Phylogenetic%20and%20functional%20diversity%20of%20aldehyde-alcohol%20dehydrogenases%20in%20microalgae.%20%3Ci%3EPlant%20Molecular%20Biology%3C%5C%2Fi%3E%2C%20%3Ci%3E105%3C%5C%2Fi%3E%284%26%23x2013%3B5%29%2C%20497%26%23x2013%3B511.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs11103-020-01105-9%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs11103-020-01105-9%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Phylogenetic%20and%20functional%20diversity%20of%20aldehyde-alcohol%20dehydrogenases%20in%20microalgae%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%22%2C%22lastName%22%3A%22van%20Lis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugi%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicolas%20J.%22%2C%22lastName%22%3A%22Tourasse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoist%22%2C%22lastName%22%3A%22Laurent%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wolfgang%22%2C%22lastName%22%3A%22Nitschke%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Vallon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ariane%22%2C%22lastName%22%3A%22Atteia%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-03%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1007%5C%2Fs11103-020-01105-9%22%2C%22ISSN%22%3A%220167-4412%2C%201573-5028%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Flink.springer.com%5C%2F10.1007%5C%2Fs11103-020-01105-9%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A07Z%22%7D%7D%2C%7B%22key%22%3A%22RICGV7MZ%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Trauchessec%20et%20al.%22%2C%22parsedDate%22%3A%222021-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ETrauchessec%2C%20M.%2C%20Hesse%2C%20A.%20M.%2C%20Kraut%2C%20A.%2C%20Berard%2C%20Y.%2C%20Herment%2C%20L.%2C%20Fortin%2C%20T.%2C%20Bruley%2C%20C.%2C%20Ferro%2C%20M.%2C%20%26amp%3B%20Manin%2C%20C.%20%282021%29.%20An%20innovative%20standard%20for%20LC%26%23x2010%3BMS%26%23x2010%3Bbased%20HCP%20profiling%20and%20accurate%20quantity%20assessment%3A%20Application%20to%20batch%20consistency%20in%20viral%20vaccine%20samples.%20%3Ci%3EPROTEOMICS%3C%5C%2Fi%3E%2C%20%3Ci%3E21%3C%5C%2Fi%3E%285%29%2C%202000152.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fpmic.202000152%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fpmic.202000152%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22An%20innovative%20standard%20for%20LC%5Cu2010MS%5Cu2010based%20HCP%20profiling%20and%20accurate%20quantity%20assessment%3A%20Application%20to%20batch%20consistency%20in%20viral%20vaccine%20samples%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathieu%22%2C%22lastName%22%3A%22Trauchessec%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne%20Marie%22%2C%22lastName%22%3A%22Hesse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Kraut%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Berard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Herment%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tanguy%22%2C%22lastName%22%3A%22Fortin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Bruley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Ferro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Catherine%22%2C%22lastName%22%3A%22Manin%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-03%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1002%5C%2Fpmic.202000152%22%2C%22ISSN%22%3A%221615-9853%2C%201615-9861%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2F10.1002%5C%2Fpmic.202000152%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A07Z%22%7D%7D%2C%7B%22key%22%3A%22G7DCWBZL%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Hseiky%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-23%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHseiky%2C%20A.%2C%20Crespo%2C%20M.%2C%20Kieffer-Jaquinod%2C%20S.%2C%20Fenaille%2C%20F.%2C%20%26amp%3B%20Pflieger%2C%20D.%20%282021%29.%20Small%20Mass%20but%20Strong%20Information%3A%20Diagnostic%20Ions%20Provide%20Crucial%20Clues%20to%20Correctly%20Identify%20Histone%20Lysine%20Modifications.%20%3Ci%3EProteomes%3C%5C%2Fi%3E%2C%20%3Ci%3E9%3C%5C%2Fi%3E%282%29%2C%2018.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fproteomes9020018%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fproteomes9020018%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Small%20Mass%20but%20Strong%20Information%3A%20Diagnostic%20Ions%20Provide%20Crucial%20Clues%20to%20Correctly%20Identify%20Histone%20Lysine%20Modifications%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alaa%22%2C%22lastName%22%3A%22Hseiky%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marion%22%2C%22lastName%22%3A%22Crespo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvie%22%2C%22lastName%22%3A%22Kieffer-Jaquinod%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois%22%2C%22lastName%22%3A%22Fenaille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Delphine%22%2C%22lastName%22%3A%22Pflieger%22%7D%5D%2C%22abstractNote%22%3A%22%281%29%20Background%3A%20The%20proteomic%20analysis%20of%20histones%20constitutes%20a%20delicate%20task%20due%20to%20the%20combination%20of%20two%20factors%3A%20slight%20variations%20in%20the%20amino%20acid%20sequences%20of%20variants%20and%20the%20multiplicity%20of%20post-translational%20modifications%20%28PTMs%29%2C%20particularly%20those%20occurring%20on%20lysine%20residues.%20%282%29%20Methods%3A%20To%20dissect%20the%20relationship%20between%20both%20aspects%2C%20we%20carefully%20evaluated%20PTM%20identification%20on%20lysine%2027%20from%20histone%20H3%20%28H3K27%29%20and%20the%20artefactual%20chemical%20modifications%20that%20may%20lead%20to%20erroneous%20PTM%20determination.%20H3K27%20is%20a%20particularly%20interesting%20example%20because%20it%20can%20bear%20a%20range%20of%20PTMs%20and%20it%20sits%20nearby%20residues%2029%20and%2031%20that%20vary%20between%20H3%20sequence%20variants.%20We%20discuss%20how%20the%20retention%20times%2C%20neutral%20losses%20and%20immonium%5C%2Fdiagnostic%20ions%20observed%20in%20the%20MS%5C%2FMS%20spectra%20of%20peptides%20bearing%20modified%20lysines%20detectable%20in%20the%20low-mass%20region%20might%20help%20validate%20the%20identification%20of%20modified%20sequences.%20%283%29%20Results%3A%20Diagnostic%20ions%20carry%20key%20information%2C%20thereby%20avoiding%20potential%20mis-identifications%20due%20to%20either%20isobaric%20PTM%20combinations%20or%20isobaric%20amino%20acid-PTM%20combinations.%20This%20also%20includes%20cases%20where%20chemical%20formylation%20or%20acetylation%20of%20peptide%20N-termini%20artefactually%20occurs%20during%20sample%20processing%20or%20simply%20in%20the%20timeframe%20of%20LC-MS%5C%2FMS%20analysis.%20Finally%2C%20in%20the%20very%20subtle%20case%20of%20positional%20isomers%20possibly%20corresponding%20to%20a%20given%20mass%20of%20lysine%20modification%2C%20the%20immonium%20and%20diagnostic%20ions%20may%20allow%20the%20identification%20of%20the%20in%20vivo%20structure.%22%2C%22date%22%3A%222021-04-23%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fproteomes9020018%22%2C%22ISSN%22%3A%222227-7382%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F2227-7382%5C%2F9%5C%2F2%5C%2F18%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A07Z%22%7D%7D%2C%7B%22key%22%3A%22IAX3N7KP%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Montillet%20et%20al.%22%2C%22parsedDate%22%3A%222021-05%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMontillet%2C%20J.-L.%2C%20Rondet%2C%20D.%2C%20Brugi%26%23xE8%3Bre%2C%20S.%2C%20Henri%2C%20P.%2C%20Rumeau%2C%20D.%2C%20Reichheld%2C%20J.-P.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Leonhardt%2C%20N.%2C%20%26amp%3B%20Rey%2C%20P.%20%282021%29.%20Plastidial%20and%20cytosolic%20thiol%20reductases%20participate%20in%20the%20control%20of%20stomatal%20functioning.%20%3Ci%3EPlant%2C%20Cell%20%26amp%3B%20Environment%3C%5C%2Fi%3E%2C%20%3Ci%3E44%3C%5C%2Fi%3E%285%29%2C%201417%26%23x2013%3B1435.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fpce.14013%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fpce.14013%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Plastidial%20and%20cytosolic%20thiol%20reductases%20participate%20in%20the%20control%20of%20stomatal%20functioning%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Luc%22%2C%22lastName%22%3A%22Montillet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Damien%22%2C%22lastName%22%3A%22Rondet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugi%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricia%22%2C%22lastName%22%3A%22Henri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominique%22%2C%22lastName%22%3A%22Rumeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Philippe%22%2C%22lastName%22%3A%22Reichheld%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nathalie%22%2C%22lastName%22%3A%22Leonhardt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pascal%22%2C%22lastName%22%3A%22Rey%22%7D%5D%2C%22abstractNote%22%3A%22Stomatal%20movements%20via%20the%20control%20of%20gas%20exchanges%20determine%20plant%20growth%20in%20relation%20to%20environmental%20stimuli%20through%20a%20complex%20signalling%20network%20involving%20reactive%20oxygen%20species%20that%20lead%20to%20post-translational%20modifications%20of%20Cys%20and%20Met%20residues%2C%20and%20alter%20protein%20activity%20and%5C%2For%20conformation.%20Thiol-reductases%20%28TRs%29%2C%20which%20include%20thioredoxins%2C%20glutaredoxins%20%28GRXs%29%20and%20peroxiredoxins%20%28PRXs%29%2C%20participate%20in%20signalling%20pathways%20through%20the%20control%20of%20Cys%20redox%20status%20in%20client%20proteins.%20Their%20involvement%20in%20stomatal%20functioning%20remains%20poorly%20characterized.%20By%20performing%20a%20mass%20spectrometry-based%20proteomic%20analysis%2C%20we%20show%20that%20numerous%20thiol%20reductases%2C%20like%20PRXs%2C%20are%20highly%20abundant%20in%20guard%20cells.%20When%20investigating%20various%20Arabidopsis%20mutants%20impaired%20in%20the%20expression%20of%20TR%20genes%2C%20no%20change%20in%20stomatal%20density%20and%20index%20was%20noticed.%20In%20optimal%20growth%20conditions%2C%20a%20line%20deficient%20in%20cytosolic%20NADPH-thioredoxin%20reductases%20displayed%20higher%20stomatal%20conductance%20and%20lower%20leaf%20temperature%20evaluated%20by%20thermal%20infrared%20imaging.%20In%20contrast%2C%20lines%20deficient%20in%20plastidial%202-CysPRXs%20or%20type-II%20GRXs%20exhibited%20compared%20to%20WT%20reduced%20conductance%20and%20warmer%20leaves%20in%20optimal%20conditions%2C%20and%20enhanced%20stomatal%20closure%20in%20epidermal%20peels%20treated%20with%20abscisic%20acid%20or%20hydrogen%20peroxide.%20Altogether%2C%20these%20data%20strongly%20support%20the%20contribution%20of%20thiol%20redox%20switches%20within%20the%20signalling%20network%20regulating%20guard%20cell%20movements%20and%20stomatal%20functioning.%22%2C%22date%22%3A%222021-05%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1111%5C%2Fpce.14013%22%2C%22ISSN%22%3A%221365-3040%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A06Z%22%7D%7D%2C%7B%22key%22%3A%22VFWI5HGS%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Chiari%20et%20al.%22%2C%22parsedDate%22%3A%222021-05%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EChiari%2C%20L.%2C%20Carpentier%2C%20P.%2C%20Kieffer-Jaquinod%2C%20S.%2C%20Gogny%2C%20A.%2C%20Perard%2C%20J.%2C%20Ravanel%2C%20S.%2C%20Cobessi%2C%20D.%2C%20M%26%23xE9%3Bnage%2C%20S.%2C%20Dumas%2C%20R.%2C%20%26amp%3B%20Hamelin%2C%20O.%20%282021%29.%20LEAFY%20protein%20crystals%20with%20a%20honeycomb%20structure%20as%20a%20platform%20for%20selective%20preparation%20of%20outstanding%20stable%20bio-hybrid%20materials.%20%3Ci%3ENanoscale%3C%5C%2Fi%3E%2C%20%3Ci%3E13%3C%5C%2Fi%3E%2819%29%2C%208901%26%23x2013%3B8908.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2FD1NR00268F%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1039%5C%2FD1NR00268F%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22LEAFY%20protein%20crystals%20with%20a%20honeycomb%20structure%20as%20a%20platform%20for%20selective%20preparation%20of%20outstanding%20stable%20bio-hybrid%20materials%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucile%22%2C%22lastName%22%3A%22Chiari%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philippe%22%2C%22lastName%22%3A%22Carpentier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvie%22%2C%22lastName%22%3A%22Kieffer-Jaquinod%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alice%22%2C%22lastName%22%3A%22Gogny%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julien%22%2C%22lastName%22%3A%22Perard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22St%5Cu00e9phane%22%2C%22lastName%22%3A%22Ravanel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Cobessi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22St%5Cu00e9phane%22%2C%22lastName%22%3A%22M%5Cu00e9nage%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Renaud%22%2C%22lastName%22%3A%22Dumas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Hamelin%22%7D%5D%2C%22abstractNote%22%3A%22Crystals%20of%20the%20LEAFY%20protein%20showing%20a%20regular%20honeycomb%20architecture%20were%20used%20as%20modular%20platform%20for%20the%20preparation%20of%20a%20highly%20robustness%20ruthenium-based%20bio-hybrid%20material%20which%20was%20fully%20characterized%20by%20spectroscopic%20means%20and%20LC-MS%20analysis.%20%5Cn%20%20%20%20%20%20%20%20%20%20%2C%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Well-organized%20protein%20assemblies%20offer%20many%20properties%20that%20justify%20their%20use%20for%20the%20design%20of%20innovative%20bionanomaterials.%20Herein%2C%20crystals%20of%20the%20oligomerization%20domain%20of%20the%20LEAFY%20protein%20from%20Ginkgo%20biloba%2C%20organized%20in%20a%20honeycomb%20architecture%2C%20were%20used%20as%20a%20modular%20platform%20for%20the%20selective%20grafting%20of%20a%20ruthenium-based%20complex.%20The%20resulting%20bio-hybrid%20crystalline%20material%20was%20fully%20characterized%20by%20UV-visible%20and%20Raman%20spectroscopy%20and%20by%20mass%20spectrometry%20and%20LC-MS%20analysis%20after%20selective%20enzymatic%20digestion.%20Interestingly%2C%20insertion%20of%20complexes%20within%20the%20tubular%20structure%20affords%20an%20impressive%20increase%20in%20stability%20of%20the%20crystals%2C%20eluding%20the%20use%20of%20stabilizing%20cross-linking%20strategies.%22%2C%22date%22%3A%222021-05%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1039%5C%2FD1NR00268F%22%2C%22ISSN%22%3A%222040-3364%2C%202040-3372%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fxlink.rsc.org%5C%2F%3FDOI%3DD1NR00268F%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A06Z%22%7D%7D%2C%7B%22key%22%3A%22JUWHIAB6%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22H%5Cu00e4rrer%20et%20al.%22%2C%22parsedDate%22%3A%222021-06%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EH%26%23xE4%3Brrer%2C%20D.%2C%20Windhorst%2C%20C.%2C%20B%26%23xF6%3Bhner%2C%20N.%2C%20Novion%20Ducassou%2C%20J.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20%26amp%3B%20Gescher%2C%20J.%20%282021%29.%20Production%20of%20acetoin%20from%20renewable%20resources%20under%20heterotrophic%20and%20mixotrophic%20conditions.%20%3Ci%3EBioresource%20Technology%3C%5C%2Fi%3E%2C%20%3Ci%3E329%3C%5C%2Fi%3E%2C%20124866.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biortech.2021.124866%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biortech.2021.124866%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Production%20of%20acetoin%20from%20renewable%20resources%20under%20heterotrophic%20and%20mixotrophic%20conditions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daniel%22%2C%22lastName%22%3A%22H%5Cu00e4rrer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Carina%22%2C%22lastName%22%3A%22Windhorst%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicola%22%2C%22lastName%22%3A%22B%5Cu00f6hner%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%22%2C%22lastName%22%3A%22Novion%20Ducassou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johannes%22%2C%22lastName%22%3A%22Gescher%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-06%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.biortech.2021.124866%22%2C%22ISSN%22%3A%2209608524%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Flinkinghub.elsevier.com%5C%2Fretrieve%5C%2Fpii%5C%2FS0960852421002054%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22BQHKBZ62%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Pallavicini%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPallavicini%2C%20G.%2C%20Gai%2C%20M.%2C%20Iegiani%2C%20G.%2C%20Berto%2C%20G.%20E.%2C%20Adrait%2C%20A.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20%26amp%3B%20Di%20Cunto%2C%20F.%20%282021%29.%20Goldberg%26%23x2013%3BShprintzen%20syndrome%20protein%20KIF1BP%20is%20a%20CITK%20interactor%20implicated%20in%20cytokinesis.%20%3Ci%3EJournal%20of%20Cell%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3E134%3C%5C%2Fi%3E%2811%29%2C%20jcs250902.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1242%5C%2Fjcs.250902%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1242%5C%2Fjcs.250902%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Goldberg%5Cu2013Shprintzen%20syndrome%20protein%20KIF1BP%20is%20a%20CITK%20interactor%20implicated%20in%20cytokinesis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gianmarco%22%2C%22lastName%22%3A%22Pallavicini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marta%22%2C%22lastName%22%3A%22Gai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giorgia%22%2C%22lastName%22%3A%22Iegiani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gaia%20Elena%22%2C%22lastName%22%3A%22Berto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annie%22%2C%22lastName%22%3A%22Adrait%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ferdinando%22%2C%22lastName%22%3A%22Di%20Cunto%22%7D%5D%2C%22abstractNote%22%3A%22ABSTRACT%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Goldberg%5Cu2013Shprintzen%20disease%20%28GOSHS%29%20is%20a%20rare%20microcephaly%20syndrome%20accompanied%20by%20intellectual%20disability%2C%20dysmorphic%20facial%20features%2C%20peripheral%20neuropathy%20and%20Hirschsprung%20disease.%20It%20is%20associated%20with%20recessive%20mutations%20in%20the%20gene%20encoding%20kinesin%20family%20member%201-binding%20protein%20%28KIF1BP%2C%20also%20known%20as%20KIFBP%29.%20The%20encoded%20protein%20regulates%20axon%20microtubules%20dynamics%2C%20kinesin%20attachment%20and%20mitochondrial%20biogenesis%2C%20but%20it%20is%20not%20clear%20how%20its%20loss%20could%20lead%20to%20microcephaly.%20We%20identified%20KIF1BP%20in%20the%20interactome%20of%20citron%20kinase%20%28CITK%2C%20also%20known%20as%20CIT%29%2C%20a%20protein%20produced%20by%20the%20primary%20hereditary%20microcephaly%2017%20%28MCPH17%29%20gene.%20KIF1BP%20and%20CITK%20interact%20under%20physiological%20conditions%20in%20mitotic%20cells.%20Similar%20to%20CITK%2C%20KIF1BP%20is%20enriched%20at%20the%20midbody%20ring%20and%20is%20required%20for%20cytokinesis.%20The%20association%20between%20KIF1BP%20and%20CITK%20can%20be%20influenced%20by%20CITK%20activity%2C%20and%20the%20two%20proteins%20may%20antagonize%20each%20other%20for%20their%20midbody%20localization.%20KIF1BP%20knockdown%20decreases%20microtubule%20stability%2C%20increases%20KIF23%20midbody%20levels%20and%20impairs%20midbody%20localization%20of%20KIF14%2C%20as%20well%20as%20of%20chromosome%20passenger%20complex.%20These%20data%20indicate%20that%20KIF1BP%20is%20a%20CITK%20interactor%20involved%20in%20midbody%20maturation%20and%20abscission%2C%20and%20suggest%20that%20cytokinesis%20failure%20may%20contribute%20to%20the%20microcephaly%20phenotype%20observed%20in%20GOSHS.%22%2C%22date%22%3A%222021-06-01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1242%5C%2Fjcs.250902%22%2C%22ISSN%22%3A%220021-9533%2C%201477-9137%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fjournals.biologists.com%5C%2Fjcs%5C%2Farticle%5C%2F134%5C%2F11%5C%2Fjcs250902%5C%2F269053%5C%2FGoldberg-Shprintzen-syndrome-protein-KIF1BP-is-a%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22CQUC48XU%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Edel%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EEdel%2C%20M.%2C%20Sturm%2C%20G.%2C%20Sturm-Richter%2C%20K.%2C%20Wagner%2C%20M.%2C%20Ducassou%2C%20J.%20N.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Horn%2C%20H.%2C%20%26amp%3B%20Gescher%2C%20J.%20%282021%29.%20Extracellular%20riboflavin%20induces%20anaerobic%20biofilm%20formation%20in%20Shewanella%20oneidensis.%20%3Ci%3EBiotechnology%20for%20Biofuels%3C%5C%2Fi%3E%2C%20%3Ci%3E14%3C%5C%2Fi%3E%281%29%2C%20130.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs13068-021-01981-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1186%5C%2Fs13068-021-01981-3%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Extracellular%20riboflavin%20induces%20anaerobic%20biofilm%20formation%20in%20Shewanella%20oneidensis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miriam%22%2C%22lastName%22%3A%22Edel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gunnar%22%2C%22lastName%22%3A%22Sturm%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Katrin%22%2C%22lastName%22%3A%22Sturm-Richter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Wagner%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%20Novion%22%2C%22lastName%22%3A%22Ducassou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Harald%22%2C%22lastName%22%3A%22Horn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johannes%22%2C%22lastName%22%3A%22Gescher%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Background%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Some%20microorganisms%20can%20respire%20with%20extracellular%20electron%20acceptors%20using%20an%20extended%20electron%20transport%20chain%20to%20the%20cell%20surface.%20This%20process%20can%20be%20applied%20in%20bioelectrochemical%20systems%20in%20which%20the%20organisms%20produce%20an%20electrical%20current%20by%20respiring%20with%20an%20anode%20as%20electron%20acceptor.%20These%20organisms%20apply%20flavin%20molecules%20as%20cofactors%20to%20facilitate%20one-electron%20transfer%20catalyzed%20by%20the%20terminal%20reductases%20and%20in%20some%20cases%20as%20endogenous%20electron%20shuttles.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Results%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20In%20the%20model%20organism%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20Shewanella%20oneidensis%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20riboflavin%20production%20and%20excretion%20trigger%20a%20specific%20biofilm%20formation%20response%20that%20is%20initiated%20at%20a%20specific%20threshold%20concentration%2C%20similar%20to%20canonical%20quorum-sensing%20molecules.%20Riboflavin-mediated%20messaging%20is%20based%20on%20the%20overexpression%20of%20the%20gene%20encoding%20the%20putrescine%20decarboxylase%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20speC%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20which%20leads%20to%20posttranscriptional%20overproduction%20of%20proteins%20involved%20in%20biofilm%20formation.%20Using%20a%20model%20of%20growth-dependent%20riboflavin%20production%20under%20batch%20and%20biofilm%20growth%20conditions%2C%20the%20number%20of%20cells%20necessary%20to%20produce%20the%20threshold%20concentration%20per%20time%20was%20deduced.%20Furthermore%2C%20our%20results%20indicate%20that%20specific%20retention%20of%20riboflavin%20in%20the%20biofilm%20matrix%20leads%20to%20localized%20concentrations%2C%20which%20by%20far%20exceed%20the%20necessary%20threshold%20value.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Conclusion%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20This%20study%20describes%20a%20new%20quorum-sensing%20mechanism%20in%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20S.%20oneidensis%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20Biofilm%20formation%20of%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20S.%20oneidensis%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20is%20induced%20by%20low%20concentrations%20of%20riboflavin%20resulting%20in%20an%20upregulation%20of%20the%20ornithine-decarboxylase%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20speC%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20The%20results%20can%20be%20applied%20for%20the%20development%20of%20strains%20catalyzing%20increased%20current%20densities%20in%20bioelectrochemical%20systems.%22%2C%22date%22%3A%222021-06-03%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1186%5C%2Fs13068-021-01981-3%22%2C%22ISSN%22%3A%221754-6834%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fbiotechnologyforbiofuels.biomedcentral.com%5C%2Farticles%5C%2F10.1186%5C%2Fs13068-021-01981-3%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A05Z%22%7D%7D%2C%7B%22key%22%3A%22Z53DHRLC%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Alfaro%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-07%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EAlfaro%2C%20J.%20A.%2C%20Bohl%26%23xE4%3Bnder%2C%20P.%2C%20Dai%2C%20M.%2C%20Filius%2C%20M.%2C%20Howard%2C%20C.%20J.%2C%20van%20Kooten%2C%20X.%20F.%2C%20Ohayon%2C%20S.%2C%20Pomorski%2C%20A.%2C%20Schmid%2C%20S.%2C%20Aksimentiev%2C%20A.%2C%20Anslyn%2C%20E.%20V.%2C%20Bedran%2C%20G.%2C%20Cao%2C%20C.%2C%20Chinappi%2C%20M.%2C%20Coyaud%2C%20E.%2C%20Dekker%2C%20C.%2C%20Dittmar%2C%20G.%2C%20Drachman%2C%20N.%2C%20Eelkema%2C%20R.%2C%20%26%23x2026%3B%20Joo%2C%20C.%20%282021%29.%20The%20emerging%20landscape%20of%20single-molecule%20protein%20sequencing%20technologies.%20%3Ci%3ENature%20Methods%3C%5C%2Fi%3E%2C%20%3Ci%3E18%3C%5C%2Fi%3E%286%29%2C%20604%26%23x2013%3B617.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41592-021-01143-1%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41592-021-01143-1%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20emerging%20landscape%20of%20single-molecule%20protein%20sequencing%20technologies%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Javier%20Antonio%22%2C%22lastName%22%3A%22Alfaro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peggy%22%2C%22lastName%22%3A%22Bohl%5Cu00e4nder%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mingjie%22%2C%22lastName%22%3A%22Dai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mike%22%2C%22lastName%22%3A%22Filius%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cecil%20J.%22%2C%22lastName%22%3A%22Howard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xander%20F.%22%2C%22lastName%22%3A%22van%20Kooten%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shilo%22%2C%22lastName%22%3A%22Ohayon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adam%22%2C%22lastName%22%3A%22Pomorski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sonja%22%2C%22lastName%22%3A%22Schmid%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aleksei%22%2C%22lastName%22%3A%22Aksimentiev%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20V.%22%2C%22lastName%22%3A%22Anslyn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Georges%22%2C%22lastName%22%3A%22Bedran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chan%22%2C%22lastName%22%3A%22Cao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mauro%22%2C%22lastName%22%3A%22Chinappi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Etienne%22%2C%22lastName%22%3A%22Coyaud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cees%22%2C%22lastName%22%3A%22Dekker%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gunnar%22%2C%22lastName%22%3A%22Dittmar%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicholas%22%2C%22lastName%22%3A%22Drachman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rienk%22%2C%22lastName%22%3A%22Eelkema%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Goodlett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S%5Cu00e9bastien%22%2C%22lastName%22%3A%22Hentz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Umesh%22%2C%22lastName%22%3A%22Kalathiya%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Neil%20L.%22%2C%22lastName%22%3A%22Kelleher%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ryan%20T.%22%2C%22lastName%22%3A%22Kelly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zvi%22%2C%22lastName%22%3A%22Kelman%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sung%20Hyun%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bernhard%22%2C%22lastName%22%3A%22Kuster%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Rodriguez-Larrea%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Stuart%22%2C%22lastName%22%3A%22Lindsay%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giovanni%22%2C%22lastName%22%3A%22Maglia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Edward%20M.%22%2C%22lastName%22%3A%22Marcotte%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22John%20P.%22%2C%22lastName%22%3A%22Marino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Masselon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Mayer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patroklos%22%2C%22lastName%22%3A%22Samaras%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kumar%22%2C%22lastName%22%3A%22Sarthak%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lusia%22%2C%22lastName%22%3A%22Sepiashvili%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Derek%22%2C%22lastName%22%3A%22Stein%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Meni%22%2C%22lastName%22%3A%22Wanunu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathias%22%2C%22lastName%22%3A%22Wilhelm%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peng%22%2C%22lastName%22%3A%22Yin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amit%22%2C%22lastName%22%3A%22Meller%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chirlmin%22%2C%22lastName%22%3A%22Joo%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-06-07%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41592-021-01143-1%22%2C%22ISSN%22%3A%221548-7091%2C%201548-7105%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41592-021-01143-1%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A04Z%22%7D%7D%2C%7B%22key%22%3A%225J9N4IKS%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Humbert%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-09%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHumbert%2C%20P.%2C%20Brennan%2C%20M.%20%26%23xC1%3B.%2C%20De%20Lima%2C%20J.%2C%20Brion%2C%20R.%2C%20Adrait%2C%20A.%2C%20Charrier%2C%20C.%2C%20Brulin%2C%20B.%2C%20Trichet%2C%20V.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Blanchard%2C%20F.%2C%20%26amp%3B%20Layrolle%2C%20P.%20%282021%29.%20Apoptotic%20mesenchymal%20stromal%20cells%20support%20osteoclastogenesis%20while%20inhibiting%20multinucleated%20giant%20cells%20formation%20in%20vitro.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2012144.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-91258-4%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-91258-4%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Apoptotic%20mesenchymal%20stromal%20cells%20support%20osteoclastogenesis%20while%20inhibiting%20multinucleated%20giant%20cells%20formation%20in%20vitro%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%22%2C%22lastName%22%3A%22Humbert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Meadhbh%20%5Cu00c1.%22%2C%22lastName%22%3A%22Brennan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julien%22%2C%22lastName%22%3A%22De%20Lima%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R%5Cu00e9gis%22%2C%22lastName%22%3A%22Brion%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annie%22%2C%22lastName%22%3A%22Adrait%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C%5Cu00e9line%22%2C%22lastName%22%3A%22Charrier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22B%5Cu00e9n%5Cu00e9dicte%22%2C%22lastName%22%3A%22Brulin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Val%5Cu00e9rie%22%2C%22lastName%22%3A%22Trichet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fr%5Cu00e9d%5Cu00e9ric%22%2C%22lastName%22%3A%22Blanchard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%22%2C%22lastName%22%3A%22Layrolle%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20In%20bone%20regeneration%20induced%20by%20the%20combination%20of%20mesenchymal%20stromal%20cells%20%28MSCs%29%20and%20calcium-phosphate%20%28CaP%29%20materials%2C%20osteoclasts%20emerge%20as%20a%20pivotal%20cell%20linking%20inflammation%20and%20bone%20formation.%20Favorable%20outcomes%20are%20observed%20despite%20short-term%20engraftments%20of%20implanted%20MSCs%2C%20highlighting%20their%20major%20paracrine%20function%20and%20the%20possible%20implication%20of%20cell%20death%20in%20modulating%20their%20secretions.%20In%20this%20work%2C%20we%20focused%20on%20the%20communication%20from%20MSCs%20towards%20osteoclasts-like%20cells%20in%20vitro.%20MSCs%20seeded%20on%20a%20CaP%20biomaterial%20or%20undergoing%20induced%20apoptosis%20produced%20a%20conditioned%20media%20favoring%20the%20development%20of%20osteoclasts%20from%20human%20CD14%2B%20monocytes.%20On%20the%20contrary%2C%20MSCs%5Cu2019%20apoptotic%20secretion%20inhibited%20the%20development%20of%20inflammatory%20multinucleated%20giant%20cells%20formed%20after%20IL-4%20stimulation.%20Components%20of%20MSCs%5Cu2019%20secretome%20before%20and%20after%20apoptotic%20stress%20were%20compared%20using%20mass%20spectrometry-based%20quantitative%20proteomics%20and%20a%20complementary%20immunoassay%20for%20major%20cytokines.%20CXCR-1%20and%20CXCR-2%20ligands%2C%20primarily%20IL-8%5C%2FCXCL-8%20but%20also%20the%20growth-regulated%20proteins%20CXCL-1%2C%20-2%20or%20-3%2C%20were%20suggested%20as%20the%20major%20players%20of%20MSCs%5Cu2019%20pro-osteoclastic%20effect.%20These%20findings%20support%20the%20hypothesis%20that%20osteoclasts%20are%20key%20players%20in%20bone%20regeneration%20and%20suggest%20that%20apoptosis%20plays%20an%20important%20role%20in%20MSCs%5Cu2019%20effectiveness.%22%2C%22date%22%3A%222021-06-09%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-91258-4%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-91258-4%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A04Z%22%7D%7D%2C%7B%22key%22%3A%222XVH7FN5%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Pipier%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-29%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPipier%2C%20A.%2C%20Devaux%2C%20A.%2C%20Lavergne%2C%20T.%2C%20Adrait%2C%20A.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Britton%2C%20S.%2C%20Calsou%2C%20P.%2C%20Riou%2C%20J.%20F.%2C%20Defrancq%2C%20E.%2C%20%26amp%3B%20Gomez%2C%20D.%20%282021%29.%20Constrained%20G4%20structures%20unveil%20topology%20specificity%20of%20known%20and%20new%20G4%20binding%20proteins.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2013469.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-92806-8%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-92806-8%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Constrained%20G4%20structures%20unveil%20topology%20specificity%20of%20known%20and%20new%20G4%20binding%20proteins%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Pipier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Devaux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T.%22%2C%22lastName%22%3A%22Lavergne%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22A.%22%2C%22lastName%22%3A%22Adrait%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Y.%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Britton%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22P.%22%2C%22lastName%22%3A%22Calsou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J.%20F.%22%2C%22lastName%22%3A%22Riou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22E.%22%2C%22lastName%22%3A%22Defrancq%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22D.%22%2C%22lastName%22%3A%22Gomez%22%7D%5D%2C%22abstractNote%22%3A%22G-quadruplexes%20%28G4%29%20are%20non-canonical%20secondary%20structures%20consisting%20in%20stacked%20tetrads%20of%20hydrogen-bonded%20guanines%20bases.%20An%20essential%20feature%20of%20G4%20is%20their%20intrinsic%20polymorphic%20nature%2C%20which%20is%20characterized%20by%20the%20equilibrium%20between%20several%20conformations%20%28also%20called%20topologies%29%20and%20the%20presence%20of%20different%20types%20of%20loops%20with%20variable%20lengths.%20In%20cells%2C%20G4%20functions%20rely%20on%20protein%20or%20enzymatic%20factors%20that%20recognize%20and%20promote%20or%20resolve%20these%20structures.%20In%20order%20to%20characterize%20new%20G4-dependent%20mechanisms%2C%20extensive%20researches%20aimed%20at%20identifying%20new%20G4%20binding%20proteins.%20Using%20G-rich%20single-stranded%20oligonucleotides%20that%20adopt%20non-controlled%20G4%20conformations%2C%20a%20large%20number%20of%20G4-binding%20proteins%20have%20been%20identified%20in%20vitro%2C%20but%20their%20specificity%20towards%20G4%20topology%20remained%20unknown.%20Constrained%20G4%20structures%20are%20biomolecular%20objects%20based%20on%20the%20use%20of%20a%20rigid%20cyclic%20peptide%20scaffold%20as%20a%20template%20for%20directing%20the%20intramolecular%20assembly%20of%20the%20anchored%20oligonucleotides%20into%20a%20single%20and%20stabilized%20G4%20topology.%20Here%2C%20using%20various%20constrained%20RNA%20or%20DNA%20G4%20as%20baits%20in%20human%20cell%20extracts%2C%20we%20establish%20the%20topology%20preference%20of%20several%20well-known%20G4-interacting%20factors.%20Moreover%2C%20we%20identify%20new%20G4-interacting%20proteins%20such%20as%20the%20NELF%20complex%20involved%20in%20the%20RNA-Pol%20II%20pausing%20mechanism%2C%20and%20we%20show%20that%20it%20impacts%20the%20clastogenic%20effect%20of%20the%20G4-ligand%20pyridostatin.%22%2C%22date%22%3A%222021-06-29%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-92806-8%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-92806-8%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A38%3A04Z%22%7D%7D%2C%7B%22key%22%3A%22AACJDW5I%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Combes%20et%20al.%22%2C%22parsedDate%22%3A%222021-07%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECombes%2C%20F.%2C%20Loux%2C%20V.%2C%20%26amp%3B%20Vandenbrouck%2C%20Y.%20%282021%29.%20for%20Differential%20Proteomics%20Using.%20In%20D.%20Cecconi%20%28Ed.%29%2C%20%3Ci%3EProteomics%20Data%20Analysis%3C%5C%2Fi%3E%20%28Vol.%202361%2C%20pp.%20179%26%23x2013%3B196%29.%20Springer%20US.%20https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2F978-1-0716-1641-3_11%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22bookSection%22%2C%22title%22%3A%22for%20Differential%20Proteomics%20Using%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Daniela%22%2C%22lastName%22%3A%22Cecconi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florence%22%2C%22lastName%22%3A%22Combes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valentin%22%2C%22lastName%22%3A%22Loux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Vandenbrouck%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22bookTitle%22%3A%22Proteomics%20Data%20Analysis%22%2C%22date%22%3A%222021-07%22%2C%22language%22%3A%22en%22%2C%22ISBN%22%3A%229781071616406%209781071616413%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Flink.springer.com%5C%2F10.1007%5C%2F978-1-0716-1641-3_11%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222022-06-23T08%3A03%3A24Z%22%7D%7D%2C%7B%22key%22%3A%226HPIEXNL%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Curien%20et%20al.%22%2C%22parsedDate%22%3A%222021-07%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECurien%2C%20G.%2C%20Lyska%2C%20D.%2C%20Guglielmino%2C%20E.%2C%20Westhoff%2C%20P.%2C%20Janetzko%2C%20J.%2C%20Tardif%2C%20M.%2C%20Hallopeau%2C%20C.%2C%20Brugi%26%23xE8%3Bre%2C%20S.%2C%20Dal%20Bo%2C%20D.%2C%20Decelle%2C%20J.%2C%20Gallet%2C%20B.%2C%20Falconet%2C%20D.%2C%20Carone%2C%20M.%2C%20Remacle%2C%20C.%2C%20Ferro%2C%20M.%2C%20Weber%2C%20A.%20P.%20M.%2C%20%26amp%3B%20Finazzi%2C%20G.%20%282021%29.%20Mixotrophic%20growth%20of%20the%20extremophile%20%3Ci%3EGaldieria%20sulphuraria%3C%5C%2Fi%3E%20reveals%20the%20flexibility%20of%20its%20carbon%20assimilation%20metabolism.%20%3Ci%3ENew%20Phytologist%3C%5C%2Fi%3E%2C%20%3Ci%3E231%3C%5C%2Fi%3E%281%29%2C%20326%26%23x2013%3B338.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.17359%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fnph.17359%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mixotrophic%20growth%20of%20the%20extremophile%20%3Ci%3EGaldieria%20sulphuraria%3C%5C%2Fi%3E%20reveals%20the%20flexibility%20of%20its%20carbon%20assimilation%20metabolism%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilles%22%2C%22lastName%22%3A%22Curien%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dagmar%22%2C%22lastName%22%3A%22Lyska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Erika%22%2C%22lastName%22%3A%22Guglielmino%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Phillip%22%2C%22lastName%22%3A%22Westhoff%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Janina%22%2C%22lastName%22%3A%22Janetzko%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marianne%22%2C%22lastName%22%3A%22Tardif%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cl%5Cu00e9ment%22%2C%22lastName%22%3A%22Hallopeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugi%5Cu00e8re%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Davide%22%2C%22lastName%22%3A%22Dal%20Bo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johan%22%2C%22lastName%22%3A%22Decelle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Benoit%22%2C%22lastName%22%3A%22Gallet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Denis%22%2C%22lastName%22%3A%22Falconet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michele%22%2C%22lastName%22%3A%22Carone%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22Remacle%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Ferro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreas%20P.M.%22%2C%22lastName%22%3A%22Weber%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giovanni%22%2C%22lastName%22%3A%22Finazzi%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-07%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1111%5C%2Fnph.17359%22%2C%22ISSN%22%3A%220028-646X%2C%201469-8137%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2F10.1111%5C%2Fnph.17359%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A13Z%22%7D%7D%2C%7B%22key%22%3A%22JCLRC86M%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Gloaguen%20et%20al.%22%2C%22parsedDate%22%3A%222021-07-02%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EGloaguen%2C%20P.%2C%20Vandenbrouck%2C%20Y.%2C%20Joyard%2C%20J.%2C%20%26amp%3B%20Curien%2C%20G.%20%282021%29.%20ChloroKB%2C%20a%20cell%20metabolism%20reconstruction%20of%20the%20model%20plant%20Arabidopsis%20thaliana.%20%3Ci%3EComptes%20Rendus.%20Biologies%3C%5C%2Fi%3E%2C%20%3Ci%3E344%3C%5C%2Fi%3E%282%29%2C%20157%26%23x2013%3B163.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5802%5C%2Fcrbiol.49%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.5802%5C%2Fcrbiol.49%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22ChloroKB%2C%20a%20cell%20metabolism%20reconstruction%20of%20the%20model%20plant%20Arabidopsis%20thaliana%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pauline%22%2C%22lastName%22%3A%22Gloaguen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Vandenbrouck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacques%22%2C%22lastName%22%3A%22Joyard%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilles%22%2C%22lastName%22%3A%22Curien%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-07-02%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.5802%5C%2Fcrbiol.49%22%2C%22ISSN%22%3A%221768-3238%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fcomptes-rendus.academie-sciences.fr%5C%2Fbiologies%5C%2Farticles%5C%2F10.5802%5C%2Fcrbiol.49%5C%2F%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A35%3A38Z%22%7D%7D%2C%7B%22key%22%3A%22KGZG9XB8%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Postic%20et%20al.%22%2C%22parsedDate%22%3A%222021-07-02%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPostic%2C%20G.%2C%20Andreani%2C%20J.%2C%20Marcoux%2C%20J.%2C%20Reys%2C%20V.%2C%20Guerois%2C%20R.%2C%20Rey%2C%20J.%2C%20Mouton-Barbosa%2C%20E.%2C%20Vandenbrouck%2C%20Y.%2C%20Cianferani%2C%20S.%2C%20Burlet-Schiltz%2C%20O.%2C%20Labesse%2C%20G.%2C%20%26amp%3B%20Tuff%26%23xE9%3Bry%2C%20P.%20%282021%29.%20Proteo3Dnet%3A%20a%20web%20server%20for%20the%20integration%20of%20structural%20information%20with%20interactomics%20data.%20%3Ci%3ENucleic%20Acids%20Research%3C%5C%2Fi%3E%2C%20%3Ci%3E49%3C%5C%2Fi%3E%28W1%29%2C%20W567%26%23x2013%3BW572.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkab332%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fnar%5C%2Fgkab332%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Proteo3Dnet%3A%20a%20web%20server%20for%20the%20integration%20of%20structural%20information%20with%20interactomics%20data%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guillaume%22%2C%22lastName%22%3A%22Postic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jessica%22%2C%22lastName%22%3A%22Andreani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julien%22%2C%22lastName%22%3A%22Marcoux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Victor%22%2C%22lastName%22%3A%22Reys%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Guerois%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julien%22%2C%22lastName%22%3A%22Rey%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Mouton-Barbosa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Vandenbrouck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sarah%22%2C%22lastName%22%3A%22Cianferani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Odile%22%2C%22lastName%22%3A%22Burlet-Schiltz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilles%22%2C%22lastName%22%3A%22Labesse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierre%22%2C%22lastName%22%3A%22Tuff%5Cu00e9ry%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20Proteo3Dnet%20is%20a%20web%20server%20dedicated%20to%20the%20analysis%20of%20mass%20spectrometry%20interactomics%20experiments.%20Given%20a%20flat%20list%20of%20proteins%2C%20its%20aim%20is%20to%20organize%20it%20in%20terms%20of%20structural%20interactions%20to%20provide%20a%20clearer%20overview%20of%20the%20data.%20This%20is%20achieved%20using%20three%20means%3A%20%28i%29%20the%20search%20for%20interologs%20with%20resolved%20structure%20available%20in%20the%20protein%20data%20bank%2C%20including%20cross-species%20remote%20homology%20search%2C%20%28ii%29%20the%20search%20for%20possibly%20weaker%20interactions%20mediated%20through%20Short%20Linear%20Motifs%20as%20predicted%20by%20ELM%5Cu2014a%20unique%20feature%20of%20Proteo3Dnet%2C%20%28iii%29%20the%20search%20for%20protein%5Cu2013protein%20interactions%20physically%20validated%20in%20the%20BioGRID%20database.%20The%20server%20then%20compiles%20this%20information%20and%20returns%20a%20graph%20of%20the%20identified%20interactions%20and%20details%20about%20the%20different%20searches.%20The%20graph%20can%20be%20interactively%20explored%20to%20understand%20the%20way%20the%20core%20complexes%20identified%20could%20interact.%20It%20can%20also%20suggest%20undetected%20partners%20to%20the%20experimentalists%2C%20or%20specific%20cases%20of%20conditionally%20exclusive%20binding.%20The%20interest%20of%20Proteo3Dnet%2C%20previously%20demonstrated%20for%20the%20difficult%20cases%20of%20the%20proteasome%20and%20pragmin%20complexes%20data%20is%2C%20here%2C%20illustrated%20in%20the%20context%20of%20yeast%20precursors%20to%20the%20small%20ribosomal%20subunits%20and%20the%20smaller%20interactome%20of%2014%5Cu20133%5Cu20133zeta%20frequent%20interactors.%20The%20Proteo3Dnet%20web%20server%20is%20accessible%20at%20http%3A%5C%2F%5C%2Fbioserv.rpbs.univ-paris-diderot.fr%5C%2Fservices%5C%2FProteo3Dnet%5C%2F.%22%2C%22date%22%3A%222021-07-02%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1093%5C%2Fnar%5C%2Fgkab332%22%2C%22ISSN%22%3A%220305-1048%2C%201362-4962%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Facademic.oup.com%5C%2Fnar%5C%2Farticle%5C%2F49%5C%2FW1%5C%2FW567%5C%2F6272410%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A13Z%22%7D%7D%2C%7B%22key%22%3A%22JWYWQY4K%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Farhat%20et%20al.%22%2C%22parsedDate%22%3A%222021-07-15%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFarhat%2C%20D.%20C.%2C%20Bowler%2C%20M.%20W.%2C%20Communie%2C%20G.%2C%20Pontier%2C%20D.%2C%20Belmudes%2C%20L.%2C%20Mas%2C%20C.%2C%20Corrao%2C%20C.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Bougdour%2C%20A.%2C%20Lagrange%2C%20T.%2C%20Hakimi%2C%20M.%2C%20%26amp%3B%20Swale%2C%20C.%20%282021%29.%20A%20plant-like%20mechanism%20coupling%20m6A%20reading%20to%20polyadenylation%20safeguards%20transcriptome%20integrity%20and%20developmental%20gene%20partitioning%20in%20Toxoplasma.%20%3Ci%3EELife%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E%2C%20e68312.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.68312%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.68312%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20plant-like%20mechanism%20coupling%20m6A%20reading%20to%20polyadenylation%20safeguards%20transcriptome%20integrity%20and%20developmental%20gene%20partitioning%20in%20Toxoplasma%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dayana%20C%22%2C%22lastName%22%3A%22Farhat%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Matthew%20W%22%2C%22lastName%22%3A%22Bowler%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guillaume%22%2C%22lastName%22%3A%22Communie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dominique%22%2C%22lastName%22%3A%22Pontier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucid%22%2C%22lastName%22%3A%22Belmudes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Mas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Corrao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandre%22%2C%22lastName%22%3A%22Bougdour%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thierry%22%2C%22lastName%22%3A%22Lagrange%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mohamed-ali%22%2C%22lastName%22%3A%22Hakimi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christopher%22%2C%22lastName%22%3A%22Swale%22%7D%5D%2C%22abstractNote%22%3A%22Correct%203%27end%20processing%20of%20mRNAs%20is%20one%20of%20the%20regulatory%20cornerstones%20of%20gene%20expression.%20In%20a%20parasite%20that%20must%20adapt%20to%20the%20regulatory%20requirements%20of%20its%20multi-host%20life%20style%2C%20there%20is%20a%20need%20to%20adopt%20additional%20means%20to%20partition%20the%20distinct%20transcriptional%20signatures%20of%20the%20closely%20and%20tandemly-arranged%20stage%20specific%20genes.%20In%20this%20study%2C%20we%20report%20our%20findings%20in%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20T.%20gondii%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20of%20an%20m6A-dependent%203%27end%20polyadenylation%20serving%20as%20a%20transcriptional%20barrier%20at%20these%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20loci%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20We%20identify%20the%20core%20polyadenylation%20complex%20within%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20T.%20gondii%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%20establish%20CPSF4%20as%20a%20reader%20for%20m6A-modified%20mRNAs%2C%20via%20a%20YTH%20domain%20within%20its%20C-terminus%2C%20a%20feature%20which%20is%20shared%20with%20plants.%20We%20bring%20evidence%20of%20the%20specificity%20of%20this%20interaction%20both%20biochemically%2C%20and%20by%20determining%20the%20crystal%20structure%20at%20high%20resolution%20of%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20T.%20gondii%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20CPSF4-YTH%20in%20complex%20with%20an%20m6A%20modified%20RNA.%20We%20show%20that%20the%20loss%20of%20m6A%2C%20both%20at%20the%20level%20of%20its%20deposition%20or%20its%20recognition%20was%20associated%20with%20an%20increase%20in%20aberrantly%20elongated%20chimeric%20mRNAs%20emanating%20from%20impaired%20transcriptional%20termination%2C%20a%20phenotype%20previously%20noticed%20in%20the%20plant%20model%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Arabidopsis%20thaliana%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20Nanopore%20direct%20RNA%20sequencing%20shows%20the%20occurrence%20of%20transcriptional%20read-through%20breaching%20into%20downstream%20repressed%20stage-specific%20genes%2C%20in%20the%20absence%20of%20either%20CPSF4%20or%20the%20m6A%20RNA%20methylase%20components%20in%20both%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20T.%20gondii%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20A.%20thaliana%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20.%20Taken%20together%2C%20our%20results%20shed%20light%20on%20an%20essential%20regulatory%20mechanism%20coupling%20the%20pathways%20of%20m6A%20metabolism%20directly%20to%20the%20cleavage%20and%20polyadenylation%20processes%2C%20one%20that%20interestingly%20seem%20to%20serve%2C%20in%20both%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20T.%20gondii%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20A.%20thaliana%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%2C%20as%20a%20guardian%20against%20aberrant%20transcriptional%20read-throughs.%22%2C%22date%22%3A%222021-07-15%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.7554%5C%2FeLife.68312%22%2C%22ISSN%22%3A%222050-084X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Felifesciences.org%5C%2Farticles%5C%2F68312%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A12Z%22%7D%7D%2C%7B%22key%22%3A%22Q8XJ5GQR%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Do%20et%20al.%22%2C%22parsedDate%22%3A%222021-07-28%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EDo%2C%20L.-D.%2C%20Moritz%2C%20C.%20P.%2C%20Mu%26%23xF1%3Biz-Castrillo%2C%20S.%2C%20Pinto%2C%20A.-L.%2C%20Tholance%2C%20Y.%2C%20Brugiere%2C%20S.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Stoevesandt%2C%20O.%2C%20Taussig%2C%20M.%20J.%2C%20Rogemond%2C%20V.%2C%20Vogrig%2C%20A.%2C%20Joubert%2C%20B.%2C%20Ferraud%2C%20K.%2C%20Camdessanch%26%23xE9%3B%2C%20J.-P.%2C%20Antoine%2C%20J.-C.%2C%20%26amp%3B%20Honnorat%2C%20J.%20%282021%29.%20Argonaute%20Autoantibodies%20as%20Biomarkers%20in%20Autoimmune%20Neurologic%20Diseases.%20%3Ci%3ENeurology%20-%20Neuroimmunology%20Neuroinflammation%3C%5C%2Fi%3E%2C%20%3Ci%3E8%3C%5C%2Fi%3E%285%29%2C%20e1032.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1212%5C%2FNXI.0000000000001032%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1212%5C%2FNXI.0000000000001032%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Argonaute%20Autoantibodies%20as%20Biomarkers%20in%20Autoimmune%20Neurologic%20Diseases%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Le-Duy%22%2C%22lastName%22%3A%22Do%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christian%20P.%22%2C%22lastName%22%3A%22Moritz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sergio%22%2C%22lastName%22%3A%22Mu%5Cu00f1iz-Castrillo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne-Laurie%22%2C%22lastName%22%3A%22Pinto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yannick%22%2C%22lastName%22%3A%22Tholance%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabine%22%2C%22lastName%22%3A%22Brugiere%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Oda%22%2C%22lastName%22%3A%22Stoevesandt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%20J.%22%2C%22lastName%22%3A%22Taussig%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22V%5Cu00e9ronique%22%2C%22lastName%22%3A%22Rogemond%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alberto%22%2C%22lastName%22%3A%22Vogrig%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bastien%22%2C%22lastName%22%3A%22Joubert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karine%22%2C%22lastName%22%3A%22Ferraud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Philippe%22%2C%22lastName%22%3A%22Camdessanch%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Christophe%22%2C%22lastName%22%3A%22Antoine%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J%5Cu00e9r%5Cu00f4me%22%2C%22lastName%22%3A%22Honnorat%22%7D%5D%2C%22abstractNote%22%3A%22Objective%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20To%20identify%20and%20characterize%20autoantibodies%20%28Abs%29%20as%20novel%20biomarkers%20for%20an%20autoimmune%20context%20in%20patients%20with%20central%20and%20peripheral%20neurologic%20diseases.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Methods%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Two%20distinct%20approaches%20%28immunoprecipitation%5C%2Fmass%20spectrometry%5Cu2013based%20proteomics%20and%20protein%20microarrays%29%20and%20patients%27%20sera%20and%20CSF%20were%20used.%20The%20specificity%20of%20the%20identified%20target%20was%20confirmed%20by%20cell-based%20assay%20%28CBA%29%20in%20856%20control%20samples.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Results%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Using%20the%202%20methods%20as%20well%20as%20sera%20and%20CSF%20of%20patients%20with%20central%20and%20peripheral%20neurologic%20involvement%2C%20we%20identified%20Abs%20against%20the%20family%20of%20Argonaute%20proteins%20%28mainly%20AGO1%20and%20AGO2%29%2C%20which%20were%20already%20reported%20in%20systemic%20autoimmunity.%20AGO-Abs%20were%20mostly%20of%20immunoglobulin%20G%201%20subclass%20and%20conformation%20dependent.%20Using%20CBA%2C%20AGO-Abs%20were%20detected%20in%2021%20patients%20with%20a%20high%20suspicion%20of%20autoimmune%20neurologic%20diseases%20%2871.4%25%20were%20women%3B%20median%20age%2057%20years%29%20and%20only%20in%204%5C%2F856%20%280.5%25%29%20controls%20analyzed%20by%20CBA%20%281%20diagnosed%20with%20small-cell%20lung%20cancer%20and%20the%20other%203%20with%20Sj%5Cu00f6gren%20syndrome%29.%20Among%20the%2021%20neurologic%20patients%20identified%2C%20the%20main%20clinical%20presentations%20were%20sensory%20neuronopathy%20%288%5C%2F21%2C%2038.1%25%29%20and%20limbic%20encephalitis%20%286%5C%2F21%2C%2028.6%25%29.%20Fourteen%20patients%20%2866.7%25%29%20had%20autoimmune%20comorbidities%20and%5C%2For%20co-occurring%20Abs%2C%20whereas%20AGO-Abs%20were%20the%20only%20autoimmune%20biomarker%20for%20the%20remaining%207%5C%2F21%20%2833.3%25%29.%20Thirteen%20%2861.9%25%29%20patients%20were%20treated%20with%20immunotherapy%3B%208%5C%2F13%20%2861.5%25%29%20improved%2C%20and%203%5C%2F13%20%2823.1%25%29%20remained%20stable%2C%20suggesting%20an%20efficacy%20of%20these%20treatments.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Conclusions%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20AGO-Abs%20might%20be%20potential%20biomarkers%20of%20autoimmunity%20in%20patients%20with%20central%20and%20peripheral%20nonparaneoplastic%20neurologic%20diseases.%20In%207%20patients%2C%20AGO-Abs%20were%20the%20only%20biomarkers%3B%20thus%2C%20their%20identification%20may%20be%20useful%20to%20suspect%20the%20autoimmune%20character%20of%20the%20neurologic%20disorder.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Classification%20of%20Evidence%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20This%20study%20provides%20Class%20III%20evidence%20that%20AGO-Abs%20are%20more%20frequent%20in%20patients%20with%20autoimmune%20neurologic%20diseases%20than%20controls.%22%2C%22date%22%3A%222021-07-28%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1212%5C%2FNXI.0000000000001032%22%2C%22ISSN%22%3A%222332-7812%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fnn.neurology.org%5C%2Flookup%5C%2Fdoi%5C%2F10.1212%5C%2FNXI.0000000000001032%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A12Z%22%7D%7D%2C%7B%22key%22%3A%22ZYU8S89V%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Notaro%20et%20al.%22%2C%22parsedDate%22%3A%222021-09%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ENotaro%2C%20A.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Belmudes%2C%20L.%2C%20Laugeri%2C%20M.%20E.%2C%20Salis%2C%20A.%2C%20Damonte%2C%20G.%2C%20Molinaro%2C%20A.%2C%20Tonetti%2C%20M.%20G.%2C%20Abergel%2C%20C.%2C%20%26amp%3B%20De%20Castro%2C%20C.%20%282021%29.%20Expanding%20the%20Occurrence%20of%20Polysaccharides%20to%20the%20Viral%20World%3A%20The%20Case%20of%20Mimivirus.%20%3Ci%3EAngewandte%20Chemie%20International%20Edition%3C%5C%2Fi%3E%2C%20%3Ci%3E60%3C%5C%2Fi%3E%2836%29%2C%2019897%26%23x2013%3B19904.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fanie.202106671%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fanie.202106671%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Expanding%20the%20Occurrence%20of%20Polysaccharides%20to%20the%20Viral%20World%3A%20The%20Case%20of%20Mimivirus%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%22%2C%22lastName%22%3A%22Notaro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucid%22%2C%22lastName%22%3A%22Belmudes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20Elena%22%2C%22lastName%22%3A%22Laugeri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annalisa%22%2C%22lastName%22%3A%22Salis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gianluca%22%2C%22lastName%22%3A%22Damonte%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antonio%22%2C%22lastName%22%3A%22Molinaro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michela%20G.%22%2C%22lastName%22%3A%22Tonetti%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chantal%22%2C%22lastName%22%3A%22Abergel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cristina%22%2C%22lastName%22%3A%22De%20Castro%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-09%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1002%5C%2Fanie.202106671%22%2C%22ISSN%22%3A%221433-7851%2C%201521-3773%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2F10.1002%5C%2Fanie.202106671%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A11Z%22%7D%7D%2C%7B%22key%22%3A%22GTMCWKKV%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Schmid%20et%20al.%22%2C%22parsedDate%22%3A%222021-09%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESchmid%2C%20F.%2C%20Novion%20Ducassou%2C%20J.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20%26amp%3B%20Gescher%2C%20J.%20%282021%29.%20Developing%20Rhodobacter%20sphaeroides%20for%20cathodic%20biopolymer%20production.%20%3Ci%3EBioresource%20Technology%3C%5C%2Fi%3E%2C%20%3Ci%3E336%3C%5C%2Fi%3E%2C%20125340.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biortech.2021.125340%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biortech.2021.125340%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Developing%20Rhodobacter%20sphaeroides%20for%20cathodic%20biopolymer%20production%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ferdinand%22%2C%22lastName%22%3A%22Schmid%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Julia%22%2C%22lastName%22%3A%22Novion%20Ducassou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johannes%22%2C%22lastName%22%3A%22Gescher%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-09%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.biortech.2021.125340%22%2C%22ISSN%22%3A%2209608524%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Flinkinghub.elsevier.com%5C%2Fretrieve%5C%2Fpii%5C%2FS0960852421006805%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A11Z%22%7D%7D%2C%7B%22key%22%3A%22CLTWQFMG%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Borges%20et%20al.%22%2C%22parsedDate%22%3A%222021-09-09%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBorges%2C%20H.%2C%20Hesse%2C%20A.-M.%2C%20Kraut%2C%20A.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Brun%2C%20V.%2C%20%26amp%3B%20Burger%2C%20T.%20%282021%29.%20Well%20Plate%20Maker%3A%20a%20user-friendly%20randomized%20block%20design%20application%20to%20limit%20batch%20effects%20in%20large-scale%20biomedical%20studies.%20%3Ci%3EBioinformatics%3C%5C%2Fi%3E%2C%20%3Ci%3E37%3C%5C%2Fi%3E%2817%29%2C%202770%26%23x2013%3B2771.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbioinformatics%5C%2Fbtab065%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fbioinformatics%5C%2Fbtab065%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Well%20Plate%20Maker%3A%20a%20user-friendly%20randomized%20block%20design%20application%20to%20limit%20batch%20effects%20in%20large-scale%20biomedical%20studies%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22H%5Cu00e9l%5Cu00e8ne%22%2C%22lastName%22%3A%22Borges%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne-Marie%22%2C%22lastName%22%3A%22Hesse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Kraut%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Virginie%22%2C%22lastName%22%3A%22Brun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Burger%22%7D%2C%7B%22creatorType%22%3A%22editor%22%2C%22firstName%22%3A%22Janet%22%2C%22lastName%22%3A%22Kelso%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Summary%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Many%20factors%20can%20influence%20results%20in%20clinical%20research%2C%20in%20particular%20bias%20in%20the%20distribution%20of%20samples%20prior%20to%20biochemical%20preparation.%20Well%20Plate%20Maker%20is%20a%20user-friendly%20application%20to%20design%20single-%20or%20multiple-well%20plate%20assays.%20It%20allows%20multiple%20group%20experiments%20to%20be%20randomized%20and%20therefore%20helps%20to%20reduce%20possible%20batch%20effects.%20Although%20primarily%20fathered%20to%20optimize%20the%20design%20of%20clinical%20sample%20analysis%20by%20high%20throughput%20mass%20spectrometry%20%28e.g.%20proteomics%20or%20metabolomics%29%2C%20it%20includes%20multiple%20options%20to%20limit%20edge-of-plate%20effects%2C%20to%20incorporate%20control%20samples%20or%20to%20limit%20cross-contamination.%20It%20thus%20fits%20the%20constraints%20of%20many%20experimental%20fields.%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Availability%20and%20implementation%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Well%20Plate%20Maker%20is%20implemented%20in%20R%20and%20available%20at%20Bioconductor%20repository%20%28https%3A%5C%2F%5C%2Fbioconductor.org%5C%2Fpackages%5C%2Fwpm%29%20under%20the%20open%20source%20Artistic%202.0%20license.%20In%20addition%20to%20classical%20scripting%2C%20it%20can%20be%20used%20through%20a%20graphical%20user%20interface%2C%20developed%20with%20Shiny%20technology.%22%2C%22date%22%3A%222021-09-09%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1093%5C%2Fbioinformatics%5C%2Fbtab065%22%2C%22ISSN%22%3A%221367-4803%2C%201460-2059%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Facademic.oup.com%5C%2Fbioinformatics%5C%2Farticle%5C%2F37%5C%2F17%5C%2F2770%5C%2F6128508%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A11Z%22%7D%7D%2C%7B%22key%22%3A%22GBVUJQER%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Fortin%20et%20al.%22%2C%22parsedDate%22%3A%222021-10-01%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFortin%2C%20T.%2C%20Vysotskyi%2C%20B.%2C%20Defoort%2C%20M.%2C%20Reynaud%2C%20A.%2C%20Lai%2C%20S.-H.%2C%20Dominguez-Medina%2C%20S.%2C%20Clement%2C%20K.%2C%20Cumaku%2C%20V.%2C%20Hentz%2C%20S.%2C%20%26amp%3B%20Masselon%2C%20C.%20%282021%29.%20A%20Nonlinear%20Model%20for%20Nano-Electro%20Mechanical%20Mass%20Sensing%20Signals%20Processing.%20%3Ci%3EIEEE%20Sensors%20Journal%3C%5C%2Fi%3E%2C%20%3Ci%3E21%3C%5C%2Fi%3E%2819%29%2C%2021852%26%23x2013%3B21861.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1109%5C%2FJSEN.2021.3103713%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1109%5C%2FJSEN.2021.3103713%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20Nonlinear%20Model%20for%20Nano-Electro%20Mechanical%20Mass%20Sensing%20Signals%20Processing%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Fortin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bogdan%22%2C%22lastName%22%3A%22Vysotskyi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martial%22%2C%22lastName%22%3A%22Defoort%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adrien%22%2C%22lastName%22%3A%22Reynaud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Szu-Hsueh%22%2C%22lastName%22%3A%22Lai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sergio%22%2C%22lastName%22%3A%22Dominguez-Medina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kavya%22%2C%22lastName%22%3A%22Clement%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vaitson%22%2C%22lastName%22%3A%22Cumaku%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sebastien%22%2C%22lastName%22%3A%22Hentz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Masselon%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-10-1%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1109%5C%2FJSEN.2021.3103713%22%2C%22ISSN%22%3A%221530-437X%2C%201558-1748%2C%202379-9153%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fieeexplore.ieee.org%5C%2Fdocument%5C%2F9509545%5C%2F%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A10Z%22%7D%7D%2C%7B%22key%22%3A%22NH2N98LG%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Pailleux%20et%20al.%22%2C%22parsedDate%22%3A%222021-10-14%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPailleux%2C%20F.%2C%20Maes%2C%20P.%2C%20Jaquinod%2C%20M.%2C%20Barthelon%2C%20J.%2C%20Darnaud%2C%20M.%2C%20Lacoste%2C%20C.%2C%20Vandenbrouck%2C%20Y.%2C%20Gilquin%2C%20B.%2C%20Louwagie%2C%20M.%2C%20Hesse%2C%20A.-M.%2C%20Kraut%2C%20A.%2C%20Garin%2C%20J.%2C%20Leroy%2C%20V.%2C%20Zarski%2C%20J.-P.%2C%20Bruley%2C%20C.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Samuel%2C%20D.%2C%20Ichai%2C%20P.%2C%20Faivre%2C%20J.%2C%20%26amp%3B%20Brun%2C%20V.%20%282021%29.%20Mass%20Spectrometry-Based%20Proteomics%20Reveal%20Alcohol%20Dehydrogenase%201B%20as%20a%20Blood%20Biomarker%20Candidate%20to%20Monitor%20Acetaminophen-Induced%20Liver%20Injury.%20%3Ci%3EInternational%20Journal%20of%20Molecular%20Sciences%3C%5C%2Fi%3E%2C%20%3Ci%3E22%3C%5C%2Fi%3E%2820%29%2C%2011071.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms222011071%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fijms222011071%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Mass%20Spectrometry-Based%20Proteomics%20Reveal%20Alcohol%20Dehydrogenase%201B%20as%20a%20Blood%20Biomarker%20Candidate%20to%20Monitor%20Acetaminophen-Induced%20Liver%20Injury%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Floriane%22%2C%22lastName%22%3A%22Pailleux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pauline%22%2C%22lastName%22%3A%22Maes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michel%22%2C%22lastName%22%3A%22Jaquinod%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Justine%22%2C%22lastName%22%3A%22Barthelon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marion%22%2C%22lastName%22%3A%22Darnaud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22Lacoste%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Vandenbrouck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beno%5Cu00eet%22%2C%22lastName%22%3A%22Gilquin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mathilde%22%2C%22lastName%22%3A%22Louwagie%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne-Marie%22%2C%22lastName%22%3A%22Hesse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Kraut%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22J%5Cu00e9r%5Cu00f4me%22%2C%22lastName%22%3A%22Garin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vincent%22%2C%22lastName%22%3A%22Leroy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Pierre%22%2C%22lastName%22%3A%22Zarski%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Bruley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Didier%22%2C%22lastName%22%3A%22Samuel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philippe%22%2C%22lastName%22%3A%22Ichai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jamila%22%2C%22lastName%22%3A%22Faivre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Virginie%22%2C%22lastName%22%3A%22Brun%22%7D%5D%2C%22abstractNote%22%3A%22Acute%20liver%20injury%20%28ALI%29%20is%20a%20severe%20disorder%20resulting%20from%20excessive%20hepatocyte%20cell%20death%2C%20and%20frequently%20caused%20by%20acetaminophen%20intoxication.%20Clinical%20management%20of%20ALI%20progression%20is%20hampered%20by%20the%20dearth%20of%20blood%20biomarkers%20available.%20In%20this%20study%2C%20a%20bioinformatics%20workflow%20was%20developed%20to%20screen%20omics%20databases%20and%20identify%20potential%20biomarkers%20for%20hepatocyte%20cell%20death.%20Then%2C%20discovery%20proteomics%20was%20harnessed%20to%20select%20from%20among%20these%20candidates%20those%20that%20were%20specifically%20detected%20in%20the%20blood%20of%20acetaminophen-induced%20ALI%20patients.%20Among%20these%20candidates%2C%20the%20isoenzyme%20alcohol%20dehydrogenase%201B%20%28ADH1B%29%20was%20massively%20leaked%20into%20the%20blood.%20To%20evaluate%20ADH1B%2C%20we%20developed%20a%20targeted%20proteomics%20assay%20and%20quantified%20ADH1B%20in%20serum%20samples%20collected%20at%20different%20times%20from%2017%20patients%20admitted%20for%20acetaminophen-induced%20ALI.%20Serum%20ADH1B%20concentrations%20increased%20markedly%20during%20the%20acute%20phase%20of%20the%20disease%2C%20and%20dropped%20to%20undetectable%20levels%20during%20recovery.%20In%20contrast%20to%20alanine%20aminotransferase%20activity%2C%20the%20rapid%20drop%20in%20circulating%20ADH1B%20concentrations%20was%20followed%20by%20an%20improvement%20in%20the%20international%20normalized%20ratio%20%28INR%29%20within%2010%5Cu201348%20h%2C%20and%20was%20associated%20with%20favorable%20outcomes.%20In%20conclusion%2C%20the%20combination%20of%20omics%20data%20exploration%20and%20proteomics%20revealed%20ADH1B%20as%20a%20new%20blood%20biomarker%20candidate%20that%20could%20be%20useful%20for%20the%20monitoring%20of%20acetaminophen-induced%20ALI.%22%2C%22date%22%3A%222021-10-14%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fijms222011071%22%2C%22ISSN%22%3A%221422-0067%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F1422-0067%5C%2F22%5C%2F20%5C%2F11071%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A10Z%22%7D%7D%2C%7B%22key%22%3A%22SEWRS3L3%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Jalabert%20et%20al.%22%2C%22parsedDate%22%3A%222021-11-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EJalabert%2C%20A.%2C%20Reininger%2C%20L.%2C%20Berger%2C%20E.%2C%20Coute%2C%20Y.%2C%20Meugnier%2C%20E.%2C%20Forterre%2C%20A.%2C%20Errazuriz-Cerda%2C%20E.%2C%20Geloen%2C%20A.%2C%20Aouadi%2C%20M.%2C%20Bouzakri%2C%20K.%2C%20Rieusset%2C%20J.%2C%20%26amp%3B%20Rome%2C%20S.%20%282021%29.%20Profiling%20of%20ob%5C%2Fob%20mice%20skeletal%20muscle%20exosome-like%20vesicles%20demonstrates%20combined%20action%20of%20miRNAs%2C%20proteins%20and%20lipids%20to%20modulate%20lipid%20homeostasis%20in%20recipient%20cells.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2021626.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-00983-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-00983-3%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Profiling%20of%20ob%5C%2Fob%20mice%20skeletal%20muscle%20exosome-like%20vesicles%20demonstrates%20combined%20action%20of%20miRNAs%2C%20proteins%20and%20lipids%20to%20modulate%20lipid%20homeostasis%20in%20recipient%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Audrey%22%2C%22lastName%22%3A%22Jalabert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Reininger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Berger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Coute%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Meugnier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexis%22%2C%22lastName%22%3A%22Forterre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elizabeth%22%2C%22lastName%22%3A%22Errazuriz-Cerda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alain%22%2C%22lastName%22%3A%22Geloen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Aouadi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Karim%22%2C%22lastName%22%3A%22Bouzakri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jennifer%22%2C%22lastName%22%3A%22Rieusset%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%22%2C%22lastName%22%3A%22Rome%22%7D%5D%2C%22abstractNote%22%3A%22We%20have%20determined%20the%20lipid%2C%20protein%20and%20miRNA%20composition%20of%20skeletal%20muscle%20%28SkM%29-released%20extracellular%20vesicles%20%28ELVs%29%20from%20Ob%5C%2Fob%20%28OB%29%20vs%20wild-type%20%28WT%29%20mice.%20The%20results%20showed%20that%20atrophic%20insulin-resistant%20OB-SkM%20released%20less%20ELVs%20than%20WT-SkM%2C%20highlighted%20by%20a%20RAB35%20decrease%20and%20an%20increase%20in%20intramuscular%20cholesterol%20content.%20Proteomic%20analyses%20of%20OB-ELVs%20revealed%20a%20group%20of%2037%20proteins%20functionally%20connected%2C%20involved%20in%20lipid%20oxidation%20and%20with%20catalytic%20activities.%20OB-ELVs%20had%20modified%20contents%20for%20phosphatidylcholine%20%28PC%2034-4%2C%20PC%2040-3%20and%20PC%2034-0%29%2C%20sphingomyelin%20%28Sm%20d18%3A1%5C%2F18%3A1%29%20and%20ceramides%20%28Cer%20d18%3A1%5C%2F18%3A0%29%20and%20were%20enriched%20in%20cholesterol%2C%20likely%20to%20alleviated%20intracellular%20accumulation.%20Surprisingly%20many%20ELV%20miRNAs%20had%20a%20nuclear%20addressing%20sequence%2C%20and%20targeted%20genes%20encoding%20proteins%20with%20nuclear%20activities.%20Interestingly%2C%20SkM-ELV%20miRNA%20did%20not%20target%20mitochondria.%20The%20most%20significant%20function%20targeted%20by%20the%207%20miRNAs%20altered%20in%20OB-ELVs%20was%20lipid%20metabolism.%20In%20agreement%2C%20OB-ELVs%20induced%20lipid%20storage%20in%20recipient%20adipocytes%20and%20increased%20lipid%20up-take%20and%20fatty%20acid%20oxidation%20in%20recipient%20muscle%20cells.%20In%20addition%2C%20OB-ELVs%20altered%20insulin-sensitivity%20and%20induced%20atrophy%20in%20muscle%20cells%2C%20reproducing%20the%20phenotype%20of%20the%20releasing%20OB%20muscles.%20These%20data%20suggest%20for%20the%20first%20time%2C%20a%20cross-talk%20between%20muscle%20cells%20and%20adipocytes%2C%20through%20the%20SkM-ELV%20route%2C%20in%20favor%20of%20adipose%20tissue%20expansion.%22%2C%22date%22%3A%222021-11-03%22%2C%22language%22%3A%22eng%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-00983-3%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A10Z%22%7D%7D%2C%7B%22key%22%3A%22JQTY5WGQ%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22H%5Cu00e4ge%20et%20al.%22%2C%22parsedDate%22%3A%222021-11-11%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EH%26%23xE4%3Bge%2C%20S.%2C%20B%26%23xFC%3Bscher%2C%20N.%2C%20Pakulska%2C%20V.%2C%20Hahn%2C%20F.%2C%20Adrait%2C%20A.%2C%20Krauter%2C%20S.%2C%20Borst%2C%20E.%20M.%2C%20Schl%26%23xF6%3Btzer-Schrehardt%2C%20U.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Plachter%2C%20B.%2C%20%26amp%3B%20Marschall%2C%20M.%20%282021%29.%20The%20Complex%20Regulatory%20Role%20of%20Cytomegalovirus%20Nuclear%20Egress%20Protein%20pUL50%20in%20the%20Production%20of%20Infectious%20Virus.%20%3Ci%3ECells%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E%2811%29%2C%203119.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells10113119%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells10113119%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22The%20Complex%20Regulatory%20Role%20of%20Cytomegalovirus%20Nuclear%20Egress%20Protein%20pUL50%20in%20the%20Production%20of%20Infectious%20Virus%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sigrun%22%2C%22lastName%22%3A%22H%5Cu00e4ge%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicole%22%2C%22lastName%22%3A%22B%5Cu00fcscher%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Victoria%22%2C%22lastName%22%3A%22Pakulska%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Friedrich%22%2C%22lastName%22%3A%22Hahn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annie%22%2C%22lastName%22%3A%22Adrait%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Steffi%22%2C%22lastName%22%3A%22Krauter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eva%20Maria%22%2C%22lastName%22%3A%22Borst%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ursula%22%2C%22lastName%22%3A%22Schl%5Cu00f6tzer-Schrehardt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bodo%22%2C%22lastName%22%3A%22Plachter%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manfred%22%2C%22lastName%22%3A%22Marschall%22%7D%5D%2C%22abstractNote%22%3A%22The%20regulation%20of%20the%20nucleocytoplasmic%20release%20of%20herpesviral%20capsids%20is%20defined%20by%20the%20process%20of%20nuclear%20egress.%20Due%20to%20their%20large%20size%2C%20nuclear%20capsids%20are%20unable%20to%20traverse%20via%20nuclear%20pores%2C%20so%20that%20herpesviruses%20evolved%20to%20develop%20a%20vesicular%20transport%20pathway%20mediating%20their%20transition%20through%20both%20leaflets%20of%20the%20nuclear%20membrane.%20This%20process%20involves%20regulatory%20proteins%2C%20which%20support%20the%20local%20distortion%20of%20the%20nuclear%20envelope.%20For%20human%20cytomegalovirus%20%28HCMV%29%2C%20the%20nuclear%20egress%20complex%20%28NEC%29%20is%20determined%20by%20the%20pUL50-pUL53%20core%20that%20initiates%20multicomponent%20assembly%20with%20NEC-associated%20proteins%20and%20capsids.%20Hereby%2C%20pUL50%20serves%20as%20a%20multi-interacting%20determinant%20that%20recruits%20several%20viral%20and%20cellular%20factors%20by%20direct%20and%20indirect%20contacts.%20Recently%2C%20we%20generated%20an%20ORF-UL50-deleted%20recombinant%20HCMV%20in%20pUL50-complementing%20cells%20and%20obtained%20first%20indications%20of%20putative%20additional%20functions%20of%20pUL50.%20In%20this%20study%2C%20we%20produced%20purified%20%5Cu0394UL50%20particles%20under%20both%20complementing%20%28%5Cu0394UL50C%29%20and%20non-complementing%20%28%5Cu0394UL50N%29%20conditions%20and%20performed%20a%20phenotypical%20characterization.%20Findings%20were%20as%20follows%3A%20%28i%29%20%5Cu0394UL50N%20particle%20preparations%20exhibited%20a%20clear%20replicative%20defect%20in%20qPCR-based%20infection%20kinetics%20compared%20to%20%5Cu0394UL50C%20particles%3B%20%28ii%29%20immuno-EM%20analysis%20of%20%5Cu0394UL50C%20did%20not%20reveal%20major%20changes%20in%20nuclear%20distribution%20of%20pUL53%20and%20lamin%20A%5C%2FC%3B%20%28iii%29%20mass%20spectrometry-based%20quantitative%20proteomics%20showed%20a%20large%20concordance%20of%20protein%20contents%20in%20the%20NIEP%20fractions%20of%20%5Cu0394UL50C%20and%20%5Cu0394UL50N%20particles%2C%20but%20virion%20fraction%20was%20close%20to%20the%20detection%20limit%20for%20%5Cu0394UL50N%3B%20%28iv%29%20confocal%20imaging%20of%20viral%20marker%20proteins%20of%20immediate%20early%20%28IE%29%20and%20later%20phases%20of%20%5Cu0394UL50N%20infection%20indicated%20a%20very%20low%20number%20of%20cells%20showing%20an%20onset%20of%20viral%20lytic%20protein%20expression%3B%20and%2C%20finally%20%28v%29%20quantitative%20measurements%20of%20encapsidated%20genomes%20provided%20evidence%20for%20a%20substantial%20reduction%20in%20the%20DNA%20contents%20in%20%5Cu0394UL50N%20compared%20to%20%5Cu0394UL50C%20particles.%20In%20summary%2C%20the%20results%20point%20to%20a%20complex%20and%20important%20regulatory%20role%20of%20the%20HCMV%20nuclear%20egress%20protein%20pUL50%20in%20the%20maturation%20of%20infectious%20virus.%22%2C%22date%22%3A%222021-11-11%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fcells10113119%22%2C%22ISSN%22%3A%222073-4409%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F2073-4409%5C%2F10%5C%2F11%5C%2F3119%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A09Z%22%7D%7D%2C%7B%22key%22%3A%22SXMKY25X%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Ropers%20et%20al.%22%2C%22parsedDate%22%3A%222021-11-19%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERopers%2C%20D.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20Faure%2C%20L.%2C%20Ferr%26%23xE9%3B%2C%20S.%2C%20Labourdette%2C%20D.%2C%20Shabani%2C%20A.%2C%20Trouilh%2C%20L.%2C%20Vasseur%2C%20P.%2C%20Corre%2C%20G.%2C%20Ferro%2C%20M.%2C%20Teste%2C%20M.-A.%2C%20Geiselmann%2C%20J.%2C%20%26amp%3B%20de%20Jong%2C%20H.%20%282021%29.%20Multiomics%20Study%20of%20Bacterial%20Growth%20Arrest%20in%20a%20Synthetic%20Biology%20Application.%20%3Ci%3EACS%20Synthetic%20Biology%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E%2811%29%2C%202910%26%23x2013%3B2926.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facssynbio.1c00115%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facssynbio.1c00115%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Multiomics%20Study%20of%20Bacterial%20Growth%20Arrest%20in%20a%20Synthetic%20Biology%20Application%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Delphine%22%2C%22lastName%22%3A%22Ropers%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22La%5Cu00ebtitia%22%2C%22lastName%22%3A%22Faure%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sabrina%22%2C%22lastName%22%3A%22Ferr%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Delphine%22%2C%22lastName%22%3A%22Labourdette%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Arieta%22%2C%22lastName%22%3A%22Shabani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lidwine%22%2C%22lastName%22%3A%22Trouilh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Perrine%22%2C%22lastName%22%3A%22Vasseur%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gw%5Cu00e9na%5Cu00eblle%22%2C%22lastName%22%3A%22Corre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Ferro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Ange%22%2C%22lastName%22%3A%22Teste%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Johannes%22%2C%22lastName%22%3A%22Geiselmann%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hidde%22%2C%22lastName%22%3A%22de%20Jong%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-11-19%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1021%5C%2Facssynbio.1c00115%22%2C%22ISSN%22%3A%222161-5063%2C%202161-5063%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fpubs.acs.org%5C%2Fdoi%5C%2F10.1021%5C%2Facssynbio.1c00115%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A09Z%22%7D%7D%2C%7B%22key%22%3A%224F8LYNZT%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Bouroumeau%20et%20al.%22%2C%22parsedDate%22%3A%222021-11-24%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBouroumeau%2C%20A.%2C%20Bussot%2C%20L.%2C%20Hamaidia%2C%20S.%2C%20Garc%26%23xEC%3Ba-Sandoval%2C%20A.%2C%20Bergan-Dahl%2C%20A.%2C%20Betton-Fraisse%2C%20P.%2C%20Duley%2C%20S.%2C%20Fournier%2C%20C.%2C%20Aucagne%2C%20R.%2C%20Adrait%2C%20A.%2C%20Cout%26%23xE9%3B%2C%20Y.%2C%20McLeer%2C%20A.%2C%20Col%2C%20E.%2C%20David-Boudet%2C%20L.%2C%20Raskovalova%2C%20T.%2C%20Jacob%2C%20M.-C.%2C%20Vettier%2C%20C.%2C%20Chevalier%2C%20S.%2C%20Carras%2C%20S.%2C%20%26%23x2026%3B%20Emadali%2C%20A.%20%282021%29.%20CYCLON%20and%20NPM1%20Cooperate%20within%20an%20Oncogenic%20Network%20Predictive%20of%20R-CHOP%20Response%20in%20DLBCL.%20%3Ci%3ECancers%3C%5C%2Fi%3E%2C%20%3Ci%3E13%3C%5C%2Fi%3E%2823%29%2C%205900.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcancers13235900%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcancers13235900%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22CYCLON%20and%20NPM1%20Cooperate%20within%20an%20Oncogenic%20Network%20Predictive%20of%20R-CHOP%20Response%20in%20DLBCL%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Antonin%22%2C%22lastName%22%3A%22Bouroumeau%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lucile%22%2C%22lastName%22%3A%22Bussot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sieme%22%2C%22lastName%22%3A%22Hamaidia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andrea%22%2C%22lastName%22%3A%22Garc%5Cu00eca-Sandoval%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anna%22%2C%22lastName%22%3A%22Bergan-Dahl%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patricia%22%2C%22lastName%22%3A%22Betton-Fraisse%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Samuel%22%2C%22lastName%22%3A%22Duley%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Cyril%22%2C%22lastName%22%3A%22Fournier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Romain%22%2C%22lastName%22%3A%22Aucagne%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annie%22%2C%22lastName%22%3A%22Adrait%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yohann%22%2C%22lastName%22%3A%22Cout%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne%22%2C%22lastName%22%3A%22McLeer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Edwige%22%2C%22lastName%22%3A%22Col%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laurence%22%2C%22lastName%22%3A%22David-Boudet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tatiana%22%2C%22lastName%22%3A%22Raskovalova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marie-Christine%22%2C%22lastName%22%3A%22Jacob%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22Vettier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Simon%22%2C%22lastName%22%3A%22Chevalier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvain%22%2C%22lastName%22%3A%22Carras%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christine%22%2C%22lastName%22%3A%22Lefebvre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Caroline%22%2C%22lastName%22%3A%22Algrin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22R%5Cu00e9my%22%2C%22lastName%22%3A%22Gressin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mary%20B.%22%2C%22lastName%22%3A%22Callanan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Herv%5Cu00e9%22%2C%22lastName%22%3A%22Sartelet%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thierry%22%2C%22lastName%22%3A%22Bonnefoix%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anouk%22%2C%22lastName%22%3A%22Emadali%22%7D%5D%2C%22abstractNote%22%3A%22R-CHOP%20immuno-chemotherapy%20significantly%20improved%20clinical%20management%20of%20diffuse%20large%20B-cell%20lymphoma%20%28DLBCL%29.%20However%2C%2030%5Cu201340%25%20of%20DLBCL%20patients%20still%20present%20a%20refractory%20disease%20or%20relapse.%20Most%20of%20the%20prognostic%20markers%20identified%20to%20date%20fail%20to%20accurately%20stratify%20high-risk%20DLBCL%20patients.%20We%20have%20previously%20shown%20that%20the%20nuclear%20protein%20CYCLON%20is%20associated%20with%20DLBCL%20disease%20progression%20and%20resistance%20to%20anti-CD20%20immunotherapy%20in%20preclinical%20models.%20We%20also%20recently%20reported%20that%20it%20also%20represents%20a%20potent%20predictor%20of%20refractory%20disease%20and%20relapse%20in%20a%20retrospective%20DLBCL%20cohort.%20However%2C%20only%20sparse%20data%20are%20available%20to%20predict%20the%20potential%20biological%20role%20of%20CYCLON%20and%20how%20it%20might%20exert%20its%20adverse%20effects%20on%20lymphoma%20cells.%20Here%2C%20we%20characterized%20the%20protein%20interaction%20network%20of%20CYCLON%2C%20connecting%20this%20protein%20to%20the%20nucleolus%2C%20RNA%20processing%2C%20MYC%20signaling%20and%20cell%20cycle%20progression.%20Among%20this%20network%2C%20NPM1%2C%20a%20nucleolar%20multi-functional%20protein%20frequently%20deregulated%20in%20cancer%2C%20emerged%20as%20another%20potential%20target%20related%20to%20treatment%20resistance%20in%20DLBCL.%20Immunohistochemistry%20evaluation%20of%20CYCLON%20and%20NPM1%20revealed%20that%20their%20co-expression%20is%20strongly%20related%20to%20inferior%20prognosis%20in%20DLBCL.%20More%20specifically%2C%20alternative%20sub-cellular%20localizations%20of%20the%20proteins%20%28extra-nucleolar%20CYCLON%20and%20pan-cellular%20NPM1%29%20represent%20independent%20predictive%20factors%20specifically%20associated%20to%20R-CHOP%20refractory%20DLBCL%20patients%2C%20which%20could%20allow%20them%20to%20be%20orientated%20towards%20risk-adapted%20or%20novel%20targeted%20therapies.%22%2C%22date%22%3A%222021-11-24%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fcancers13235900%22%2C%22ISSN%22%3A%222072-6694%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F2072-6694%5C%2F13%5C%2F23%5C%2F5900%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A09Z%22%7D%7D%2C%7B%22key%22%3A%22DNT3HAPL%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Clement%20et%20al.%22%2C%22parsedDate%22%3A%222021-12%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EClement%2C%20K.%2C%20Reynaud%2C%20A.%2C%20Defoort%2C%20M.%2C%20Vysotskyi%2C%20B.%2C%20Fortin%2C%20T.%2C%20Lai%2C%20S.-H.%2C%20%26%23xC7%3Bumaku%2C%20V.%2C%20Dominguez-Medina%2C%20S.%2C%20Hentz%2C%20S.%2C%20%26amp%3B%20Masselon%2C%20C.%20%282021%29.%20Requirements%20and%20attributes%20of%20nano-resonator%20mass%20spectrometry%20for%20the%20analysis%20of%20intact%20viral%20particles.%20%3Ci%3EAnalytical%20and%20Bioanalytical%20Chemistry%3C%5C%2Fi%3E%2C%20%3Ci%3E413%3C%5C%2Fi%3E%2829%29%2C%207147%26%23x2013%3B7156.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00216-021-03511-4%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs00216-021-03511-4%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Requirements%20and%20attributes%20of%20nano-resonator%20mass%20spectrometry%20for%20the%20analysis%20of%20intact%20viral%20particles%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kavya%22%2C%22lastName%22%3A%22Clement%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Adrien%22%2C%22lastName%22%3A%22Reynaud%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Martial%22%2C%22lastName%22%3A%22Defoort%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bogdan%22%2C%22lastName%22%3A%22Vysotskyi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Fortin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Szu-Hsueh%22%2C%22lastName%22%3A%22Lai%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vaitson%22%2C%22lastName%22%3A%22%5Cu00c7umaku%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sergio%22%2C%22lastName%22%3A%22Dominguez-Medina%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S%5Cu00e9bastien%22%2C%22lastName%22%3A%22Hentz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Masselon%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-12%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1007%5C%2Fs00216-021-03511-4%22%2C%22ISSN%22%3A%221618-2642%2C%201618-2650%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Flink.springer.com%5C%2F10.1007%5C%2Fs00216-021-03511-4%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A08Z%22%7D%7D%2C%7B%22key%22%3A%222RSGZJ8E%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Imbert%20et%20al.%22%2C%22parsedDate%22%3A%222021-12-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EImbert%2C%20A.%2C%20Rompais%2C%20M.%2C%20Selloum%2C%20M.%2C%20Castelli%2C%20F.%2C%20Mouton-Barbosa%2C%20E.%2C%20Brandolini-Bunlon%2C%20M.%2C%20Chu-Van%2C%20E.%2C%20Joly%2C%20C.%2C%20Hirschler%2C%20A.%2C%20Roger%2C%20P.%2C%20Burger%2C%20T.%2C%20Leblanc%2C%20S.%2C%20Sorg%2C%20T.%2C%20Ouzia%2C%20S.%2C%20Vandenbrouck%2C%20Y.%2C%20M%26%23xE9%3Bdigue%2C%20C.%2C%20Junot%2C%20C.%2C%20Ferro%2C%20M.%2C%20Pujos-Guillot%2C%20E.%2C%20%26%23x2026%3B%20Th%26%23xE9%3Bvenot%2C%20E.%20A.%20%282021%29.%20ProMetIS%2C%20deep%20phenotyping%20of%20mouse%20models%20by%20combined%20proteomics%20and%20metabolomics%20analysis.%20%3Ci%3EScientific%20Data%3C%5C%2Fi%3E%2C%20%3Ci%3E8%3C%5C%2Fi%3E%281%29%2C%20311.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41597-021-01095-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41597-021-01095-3%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22ProMetIS%2C%20deep%20phenotyping%20of%20mouse%20models%20by%20combined%20proteomics%20and%20metabolomics%20analysis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alyssa%22%2C%22lastName%22%3A%22Imbert%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Magali%22%2C%22lastName%22%3A%22Rompais%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mohammed%22%2C%22lastName%22%3A%22Selloum%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florence%22%2C%22lastName%22%3A%22Castelli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emmanuelle%22%2C%22lastName%22%3A%22Mouton-Barbosa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marion%22%2C%22lastName%22%3A%22Brandolini-Bunlon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emeline%22%2C%22lastName%22%3A%22Chu-Van%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Joly%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aur%5Cu00e9lie%22%2C%22lastName%22%3A%22Hirschler%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Pierrick%22%2C%22lastName%22%3A%22Roger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thomas%22%2C%22lastName%22%3A%22Burger%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sophie%22%2C%22lastName%22%3A%22Leblanc%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tania%22%2C%22lastName%22%3A%22Sorg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sadia%22%2C%22lastName%22%3A%22Ouzia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Vandenbrouck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claudine%22%2C%22lastName%22%3A%22M%5Cu00e9digue%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Junot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Myriam%22%2C%22lastName%22%3A%22Ferro%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Estelle%22%2C%22lastName%22%3A%22Pujos-Guillot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne%20Gonzalez%22%2C%22lastName%22%3A%22de%20Peredo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Fran%5Cu00e7ois%22%2C%22lastName%22%3A%22Fenaille%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christine%22%2C%22lastName%22%3A%22Carapito%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yann%22%2C%22lastName%22%3A%22Herault%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Etienne%20A.%22%2C%22lastName%22%3A%22Th%5Cu00e9venot%22%7D%5D%2C%22abstractNote%22%3A%22Abstract%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Genes%20are%20pleiotropic%20and%20getting%20a%20better%20knowledge%20of%20their%20function%20requires%20a%20comprehensive%20characterization%20of%20their%20mutants.%20Here%2C%20we%20generated%20multi-level%20data%20combining%20phenomic%2C%20proteomic%20and%20metabolomic%20acquisitions%20from%20plasma%20and%20liver%20tissues%20of%20two%20C57BL%5C%2F6%5Cu2009N%20mouse%20models%20lacking%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Lat%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28linker%20for%20activation%20of%20T%20cells%29%20and%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Mx2%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20%28MX%20dynamin-like%20GTPase%202%29%20genes%2C%20respectively.%20Our%20dataset%20consists%20of%209%20assays%20%281%20preclinical%2C%202%20proteomics%20and%206%20metabolomics%29%20generated%20with%20a%20fully%20non-targeted%20and%20standardized%20approach.%20The%20data%20and%20processing%20code%20are%20publicly%20available%20in%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20ProMetIS%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20R%20package%20to%20ensure%20accessibility%2C%20interoperability%2C%20and%20reusability.%20The%20dataset%20thus%20provides%20unique%20molecular%20information%20about%20the%20physiological%20role%20of%20the%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Lat%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20and%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Mx2%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20genes.%20Furthermore%2C%20the%20protocols%20described%20herein%20can%20be%20easily%20extended%20to%20a%20larger%20number%20of%20individuals%20and%20tissues.%20Finally%2C%20this%20resource%20will%20be%20of%20great%20interest%20to%20develop%20new%20bioinformatic%20and%20biostatistic%20methods%20for%20multi-omics%20data%20integration.%22%2C%22date%22%3A%222021-12-03%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41597-021-01095-3%22%2C%22ISSN%22%3A%222052-4463%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41597-021-01095-3%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A35%3A24Z%22%7D%7D%2C%7B%22key%22%3A%22NVGD4NS9%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Boulan%20et%20al.%22%2C%22parsedDate%22%3A%222021-12-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBoulan%2C%20B.%2C%20Ravanello%2C%20C.%2C%20Peyrel%2C%20A.%2C%20Bosc%2C%20C.%2C%20Delphin%2C%20C.%2C%20Appaix%2C%20F.%2C%20Denarier%2C%20E.%2C%20Kraut%2C%20A.%2C%20Jaquier-Sarlin%2C%20M.%2C%20Fournier%2C%20A.%2C%20Andrieux%2C%20A.%2C%20Gory-Faur%26%23xE9%3B%2C%20S.%2C%20%26amp%3B%20Deloulme%2C%20J.-C.%20%282021%29.%20CRMP4-mediated%20fornix%20development%20involves%20semaphorin-3E%20signaling%20pathway.%20%3Ci%3EELife%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E%2C%20e70361.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.70361%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.7554%5C%2FeLife.70361%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22CRMP4-mediated%20fornix%20development%20involves%20semaphorin-3E%20signaling%20pathway%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Beno%5Cu00eet%22%2C%22lastName%22%3A%22Boulan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%22%2C%22lastName%22%3A%22Ravanello%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amandine%22%2C%22lastName%22%3A%22Peyrel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christophe%22%2C%22lastName%22%3A%22Bosc%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christian%22%2C%22lastName%22%3A%22Delphin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Florence%22%2C%22lastName%22%3A%22Appaix%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%22%2C%22lastName%22%3A%22Denarier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alexandra%22%2C%22lastName%22%3A%22Kraut%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Muriel%22%2C%22lastName%22%3A%22Jaquier-Sarlin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Alyson%22%2C%22lastName%22%3A%22Fournier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annie%22%2C%22lastName%22%3A%22Andrieux%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sylvie%22%2C%22lastName%22%3A%22Gory-Faur%5Cu00e9%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jean-Christophe%22%2C%22lastName%22%3A%22Deloulme%22%7D%5D%2C%22abstractNote%22%3A%22Neurodevelopmental%20axonal%20pathfinding%20plays%20a%20central%20role%20in%20correct%20brain%20wiring%20and%20subsequent%20cognitive%20abilities.%20Within%20the%20growth%20cone%2C%20various%20intracellular%20effectors%20transduce%20axonal%20guidance%20signals%20by%20remodeling%20the%20cytoskeleton.%20Semaphorin-3E%20%28Sema3E%29%20is%20a%20guidance%20cue%20implicated%20in%20development%20of%20the%20fornix%2C%20a%20neuronal%20tract%20connecting%20the%20hippocampus%20to%20the%20hypothalamus.%20Microtubule-Associated%20Protein%206%20%28MAP6%29%20has%20been%20shown%20to%20be%20involved%20in%20the%20Sema3E%20growth-promoting%20signaling%20pathway.%20In%20this%20study%2C%20we%20identified%20the%20Collapsin%20Response%20Mediator%20Protein%204%20%28CRMP4%29%20as%20a%20MAP6%20partner%20and%20a%20crucial%20effector%20in%20Sema3E%20growth-promoting%20activity.%20CRMP4-KO%20mice%20displayed%20abnormal%20fornix%20development%20reminiscent%20of%20that%20observed%20in%20Sema3E-KO%20mice.%20CRMP4%20was%20shown%20to%20interact%20with%20the%20Sema3E%20tripartite%20receptor%20complex%20within%20Detergent-%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Resistant%20%5Cn%20%20%20%20%20%20%20%20%20%20%20%20%20%20Membrane%20%28DRM%29%20domains%2C%20and%20DRM%20domain%20integrity%20was%20required%20to%20transduce%20Sema3E%20signaling%20through%20the%20Akt%5C%2FGSK3%20pathway.%20Finally%2C%20we%20showed%20that%20the%20cytoskeleton-binding%20domain%20of%20CRMP4%20is%20required%20for%20Sema3E%27s%20growth-promoting%20activity%2C%20suggesting%20that%20CRMP4%20plays%20a%20role%20at%20the%20interface%20between%20Sema3E%20receptors%2C%20located%20in%20DRM%20domains%2C%20and%20the%20cytoskeleton%20network.%20As%20the%20fornix%20is%20affected%20in%20many%20psychiatric%20diseases%2C%20such%20as%20schizophrenia%2C%20our%20results%20provide%20new%20insights%20to%20better%20understand%20the%20neurodevelopmental%20components%20of%20these%20diseases.%22%2C%22date%22%3A%222021-12-03%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.7554%5C%2FeLife.70361%22%2C%22ISSN%22%3A%222050-084X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Felifesciences.org%5C%2Farticles%5C%2F70361%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A08Z%22%7D%7D%2C%7B%22key%22%3A%223BS6E2MT%22%2C%22library%22%3A%7B%22id%22%3A238648%7D%2C%22meta%22%3A%7B%22lastModifiedByUser%22%3A%7B%22id%22%3A1535877%2C%22username%22%3A%22Christophe%20Bruley%22%2C%22name%22%3A%22Christophe%20Bruley%22%2C%22links%22%3A%7B%22alternate%22%3A%7B%22href%22%3A%22https%3A%5C%2F%5C%2Fwww.zotero.org%5C%2Fchristophe_bruley%22%2C%22type%22%3A%22text%5C%2Fhtml%22%7D%7D%7D%2C%22creatorSummary%22%3A%22Deutsch%20et%20al.%22%2C%22parsedDate%22%3A%222021-12-03%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EDeutsch%2C%20E.%20W.%2C%20Omenn%2C%20G.%20S.%2C%20Sun%2C%20Z.%2C%20Maes%2C%20M.%2C%20Pernemalm%2C%20M.%2C%20Palaniappan%2C%20K.%20K.%2C%20Letunica%2C%20N.%2C%20Vandenbrouck%2C%20Y.%2C%20Brun%2C%20V.%2C%20Tao%2C%20S.%2C%20Yu%2C%20X.%2C%20Geyer%2C%20P.%20E.%2C%20Ignjatovic%2C%20V.%2C%20Moritz%2C%20R.%20L.%2C%20%26amp%3B%20Schwenk%2C%20J.%20M.%20%282021%29.%20Advances%20and%20Utility%20of%20the%20Human%20Plasma%20Proteome.%20%3Ci%3EJournal%20of%20Proteome%20Research%3C%5C%2Fi%3E%2C%20%3Ci%3E20%3C%5C%2Fi%3E%2812%29%2C%205241%26%23x2013%3B5263.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jproteome.1c00657%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facs.jproteome.1c00657%3C%5C%2Fa%3E%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Advances%20and%20Utility%20of%20the%20Human%20Plasma%20Proteome%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eric%20W.%22%2C%22lastName%22%3A%22Deutsch%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gilbert%20S.%22%2C%22lastName%22%3A%22Omenn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhi%22%2C%22lastName%22%3A%22Sun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michal%22%2C%22lastName%22%3A%22Maes%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Pernemalm%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Krishnan%20K.%22%2C%22lastName%22%3A%22Palaniappan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Natasha%22%2C%22lastName%22%3A%22Letunica%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yves%22%2C%22lastName%22%3A%22Vandenbrouck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Virginie%22%2C%22lastName%22%3A%22Brun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheng-ce%22%2C%22lastName%22%3A%22Tao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaobo%22%2C%22lastName%22%3A%22Yu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Philipp%20E.%22%2C%22lastName%22%3A%22Geyer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vera%22%2C%22lastName%22%3A%22Ignjatovic%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Robert%20L.%22%2C%22lastName%22%3A%22Moritz%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jochen%20M.%22%2C%22lastName%22%3A%22Schwenk%22%7D%5D%2C%22abstractNote%22%3A%22%22%2C%22date%22%3A%222021-12-03%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1021%5C%2Facs.jproteome.1c00657%22%2C%22ISSN%22%3A%221535-3893%2C%201535-3907%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fpubs.acs.org%5C%2Fdoi%5C%2F10.1021%5C%2Facs.jproteome.1c00657%22%2C%22collections%22%3A%5B%22VIGCK6G3%22%5D%2C%22dateModified%22%3A%222024-06-04T06%3A37%3A08Z%22%7D%7D%5D%7D
Takata-Tsuji, F., Chounlamountri, N., Do, L.-D., Philippot, C., Novion Ducassou, J., Couté, Y., Ben Achour, S., Honnorat, J., Place, C., & Pascual, O. (2021). Microglia modulate gliotransmission through the regulation of VAMP2 proteins in astrocytes. Glia, 69(1), 61–72. https://doi.org/10.1002/glia.23884
Adrait, A., Dumonceau, J., Delhaye, M., Annessi‐Ramseyer, I., Frossard, J., Couté, Y., & Farina, A. (2021). Liquid Biopsy of Bile based on Targeted Mass Spectrometry for the Diagnosis of Malignant Biliary Strictures. Clinical and Translational Science, 14(1), 148–152. https://doi.org/10.1111/cts.12890
Felix, J., Siebert, C., Ducassou, J. N., Nigou, J., Garcia, P. S., Fraudeau, A., Huard, K., Mas, C., Brochier-Armanet, C., Couté, Y., Gutsche, I., & Renesto, P. (2021). Structural and functional analysis of the Francisella lysine decarboxylase as a key actor in oxidative stress resistance. Scientific Reports, 11(1), 972. https://doi.org/10.1038/s41598-020-79611-5
Kerjouan, A., Boyault, C., Oddou, C., Hiriart-Bryant, E., Grichine, A., Kraut, A., Pezet, M., Balland, M., Faurobert, E., Bonnet, I., Coute, Y., Fourcade, B., Albiges-Rizo, C., & Destaing, O. (2021). Control of SRC molecular dynamics encodes distinct cytoskeletal responses by specifying signaling pathway usage. Journal of Cell Science, 134(2), jcs254599. https://doi.org/10.1242/jcs.254599
Gilquin, B., Cubizolles, M., Den Dulk, R., Revol-Cavalier, F., Alessio, M., Goujon, C.-E., Echampard, C., Arrizabalaga, G., Adrait, A., Louwagie, M., Laurent, P., Navarro, F. P., Couté, Y., Cosnier, M.-L., & Brun, V. (2021). PepS: An Innovative Microfluidic Device for Bedside Whole Blood Processing before Plasma Proteomics Analyses. Analytical Chemistry, 93(2), 683–690. https://doi.org/10.1021/acs.analchem.0c02270
Rayapuram, N., Jarad, M., Alhoraibi, H. M., Bigeard, J., Abulfaraj, A. A., Völz, R., Mariappan, K. G., Almeida-Trapp, M., Schlöffel, M., Lastrucci, E., Bonhomme, L., Gust, A. A., Mithöfer, A., Arold, S. T., Pflieger, D., & Hirt, H. (2021). Chromatin phosphoproteomics unravels a function for AT-hook motif nuclear localized protein AHL13 in PAMP-triggered immunity. Proceedings of the National Academy of Sciences, 118(3), e2004670118. https://doi.org/10.1073/pnas.2004670118
Permiakova, O., Guibert, R., Kraut, A., Fortin, T., Hesse, A.-M., & Burger, T. (2021). CHICKN: extraction of peptide chromatographic elution profiles from large scale mass spectrometry data by means of Wasserstein compressive hierarchical cluster analysis. BMC Bioinformatics, 22(1), 68. https://doi.org/10.1186/s12859-021-03969-0
Vellino, S., Oddou, C., Rivier, P., Boyault, C., Hiriart-Bryant, E., Kraut, A., Martin, R., Coute, Y., Knölker, H.-J., Valverde, M. A., Albigès-Rizo, C., & Destaing, O. (2021). Cross-talk between the calcium channel TRPV4 and reactive oxygen species interlocks adhesive and degradative functions of invadosomes. Journal of Cell Biology, 220(2), e201910079. https://doi.org/10.1083/jcb.201910079
van Lis, R., Couté, Y., Brugière, S., Tourasse, N. J., Laurent, B., Nitschke, W., Vallon, O., & Atteia, A. (2021). Phylogenetic and functional diversity of aldehyde-alcohol dehydrogenases in microalgae. Plant Molecular Biology, 105(4–5), 497–511. https://doi.org/10.1007/s11103-020-01105-9
Trauchessec, M., Hesse, A. M., Kraut, A., Berard, Y., Herment, L., Fortin, T., Bruley, C., Ferro, M., & Manin, C. (2021). An innovative standard for LC‐MS‐based HCP profiling and accurate quantity assessment: Application to batch consistency in viral vaccine samples. PROTEOMICS, 21(5), 2000152. https://doi.org/10.1002/pmic.202000152
Hseiky, A., Crespo, M., Kieffer-Jaquinod, S., Fenaille, F., & Pflieger, D. (2021). Small Mass but Strong Information: Diagnostic Ions Provide Crucial Clues to Correctly Identify Histone Lysine Modifications. Proteomes, 9(2), 18. https://doi.org/10.3390/proteomes9020018
Montillet, J.-L., Rondet, D., Brugière, S., Henri, P., Rumeau, D., Reichheld, J.-P., Couté, Y., Leonhardt, N., & Rey, P. (2021). Plastidial and cytosolic thiol reductases participate in the control of stomatal functioning. Plant, Cell & Environment, 44(5), 1417–1435. https://doi.org/10.1111/pce.14013
Chiari, L., Carpentier, P., Kieffer-Jaquinod, S., Gogny, A., Perard, J., Ravanel, S., Cobessi, D., Ménage, S., Dumas, R., & Hamelin, O. (2021). LEAFY protein crystals with a honeycomb structure as a platform for selective preparation of outstanding stable bio-hybrid materials. Nanoscale, 13(19), 8901–8908. https://doi.org/10.1039/D1NR00268F
Härrer, D., Windhorst, C., Böhner, N., Novion Ducassou, J., Couté, Y., & Gescher, J. (2021). Production of acetoin from renewable resources under heterotrophic and mixotrophic conditions. Bioresource Technology, 329, 124866. https://doi.org/10.1016/j.biortech.2021.124866
Pallavicini, G., Gai, M., Iegiani, G., Berto, G. E., Adrait, A., Couté, Y., & Di Cunto, F. (2021). Goldberg–Shprintzen syndrome protein KIF1BP is a CITK interactor implicated in cytokinesis. Journal of Cell Science, 134(11), jcs250902. https://doi.org/10.1242/jcs.250902
Edel, M., Sturm, G., Sturm-Richter, K., Wagner, M., Ducassou, J. N., Couté, Y., Horn, H., & Gescher, J. (2021). Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis. Biotechnology for Biofuels, 14(1), 130. https://doi.org/10.1186/s13068-021-01981-3
Alfaro, J. A., Bohländer, P., Dai, M., Filius, M., Howard, C. J., van Kooten, X. F., Ohayon, S., Pomorski, A., Schmid, S., Aksimentiev, A., Anslyn, E. V., Bedran, G., Cao, C., Chinappi, M., Coyaud, E., Dekker, C., Dittmar, G., Drachman, N., Eelkema, R., … Joo, C. (2021). The emerging landscape of single-molecule protein sequencing technologies. Nature Methods, 18(6), 604–617. https://doi.org/10.1038/s41592-021-01143-1
Humbert, P., Brennan, M. Á., De Lima, J., Brion, R., Adrait, A., Charrier, C., Brulin, B., Trichet, V., Couté, Y., Blanchard, F., & Layrolle, P. (2021). Apoptotic mesenchymal stromal cells support osteoclastogenesis while inhibiting multinucleated giant cells formation in vitro. Scientific Reports, 11(1), 12144. https://doi.org/10.1038/s41598-021-91258-4
Pipier, A., Devaux, A., Lavergne, T., Adrait, A., Couté, Y., Britton, S., Calsou, P., Riou, J. F., Defrancq, E., & Gomez, D. (2021). Constrained G4 structures unveil topology specificity of known and new G4 binding proteins. Scientific Reports, 11(1), 13469. https://doi.org/10.1038/s41598-021-92806-8
Combes, F., Loux, V., & Vandenbrouck, Y. (2021). for Differential Proteomics Using. In D. Cecconi (Ed.), Proteomics Data Analysis (Vol. 2361, pp. 179–196). Springer US. https://doi.org/10.1007/978-1-0716-1641-3_11
Curien, G., Lyska, D., Guglielmino, E., Westhoff, P., Janetzko, J., Tardif, M., Hallopeau, C., Brugière, S., Dal Bo, D., Decelle, J., Gallet, B., Falconet, D., Carone, M., Remacle, C., Ferro, M., Weber, A. P. M., & Finazzi, G. (2021). Mixotrophic growth of the extremophile Galdieria sulphuraria reveals the flexibility of its carbon assimilation metabolism. New Phytologist, 231(1), 326–338. https://doi.org/10.1111/nph.17359
Gloaguen, P., Vandenbrouck, Y., Joyard, J., & Curien, G. (2021). ChloroKB, a cell metabolism reconstruction of the model plant Arabidopsis thaliana. Comptes Rendus. Biologies, 344(2), 157–163. https://doi.org/10.5802/crbiol.49
Postic, G., Andreani, J., Marcoux, J., Reys, V., Guerois, R., Rey, J., Mouton-Barbosa, E., Vandenbrouck, Y., Cianferani, S., Burlet-Schiltz, O., Labesse, G., & Tufféry, P. (2021). Proteo3Dnet: a web server for the integration of structural information with interactomics data. Nucleic Acids Research, 49(W1), W567–W572. https://doi.org/10.1093/nar/gkab332
Farhat, D. C., Bowler, M. W., Communie, G., Pontier, D., Belmudes, L., Mas, C., Corrao, C., Couté, Y., Bougdour, A., Lagrange, T., Hakimi, M., & Swale, C. (2021). A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental gene partitioning in Toxoplasma. ELife, 10, e68312. https://doi.org/10.7554/eLife.68312
Do, L.-D., Moritz, C. P., Muñiz-Castrillo, S., Pinto, A.-L., Tholance, Y., Brugiere, S., Couté, Y., Stoevesandt, O., Taussig, M. J., Rogemond, V., Vogrig, A., Joubert, B., Ferraud, K., Camdessanché, J.-P., Antoine, J.-C., & Honnorat, J. (2021). Argonaute Autoantibodies as Biomarkers in Autoimmune Neurologic Diseases. Neurology - Neuroimmunology Neuroinflammation, 8(5), e1032. https://doi.org/10.1212/NXI.0000000000001032
Notaro, A., Couté, Y., Belmudes, L., Laugeri, M. E., Salis, A., Damonte, G., Molinaro, A., Tonetti, M. G., Abergel, C., & De Castro, C. (2021). Expanding the Occurrence of Polysaccharides to the Viral World: The Case of Mimivirus. Angewandte Chemie International Edition, 60(36), 19897–19904. https://doi.org/10.1002/anie.202106671
Schmid, F., Novion Ducassou, J., Couté, Y., & Gescher, J. (2021). Developing Rhodobacter sphaeroides for cathodic biopolymer production. Bioresource Technology, 336, 125340. https://doi.org/10.1016/j.biortech.2021.125340
Borges, H., Hesse, A.-M., Kraut, A., Couté, Y., Brun, V., & Burger, T. (2021). Well Plate Maker: a user-friendly randomized block design application to limit batch effects in large-scale biomedical studies. Bioinformatics, 37(17), 2770–2771. https://doi.org/10.1093/bioinformatics/btab065
Fortin, T., Vysotskyi, B., Defoort, M., Reynaud, A., Lai, S.-H., Dominguez-Medina, S., Clement, K., Cumaku, V., Hentz, S., & Masselon, C. (2021). A Nonlinear Model for Nano-Electro Mechanical Mass Sensing Signals Processing. IEEE Sensors Journal, 21(19), 21852–21861. https://doi.org/10.1109/JSEN.2021.3103713
Pailleux, F., Maes, P., Jaquinod, M., Barthelon, J., Darnaud, M., Lacoste, C., Vandenbrouck, Y., Gilquin, B., Louwagie, M., Hesse, A.-M., Kraut, A., Garin, J., Leroy, V., Zarski, J.-P., Bruley, C., Couté, Y., Samuel, D., Ichai, P., Faivre, J., & Brun, V. (2021). Mass Spectrometry-Based Proteomics Reveal Alcohol Dehydrogenase 1B as a Blood Biomarker Candidate to Monitor Acetaminophen-Induced Liver Injury. International Journal of Molecular Sciences, 22(20), 11071. https://doi.org/10.3390/ijms222011071
Jalabert, A., Reininger, L., Berger, E., Coute, Y., Meugnier, E., Forterre, A., Errazuriz-Cerda, E., Geloen, A., Aouadi, M., Bouzakri, K., Rieusset, J., & Rome, S. (2021). Profiling of ob/ob mice skeletal muscle exosome-like vesicles demonstrates combined action of miRNAs, proteins and lipids to modulate lipid homeostasis in recipient cells. Scientific Reports, 11(1), 21626. https://doi.org/10.1038/s41598-021-00983-3
Häge, S., Büscher, N., Pakulska, V., Hahn, F., Adrait, A., Krauter, S., Borst, E. M., Schlötzer-Schrehardt, U., Couté, Y., Plachter, B., & Marschall, M. (2021). The Complex Regulatory Role of Cytomegalovirus Nuclear Egress Protein pUL50 in the Production of Infectious Virus. Cells, 10(11), 3119. https://doi.org/10.3390/cells10113119
Ropers, D., Couté, Y., Faure, L., Ferré, S., Labourdette, D., Shabani, A., Trouilh, L., Vasseur, P., Corre, G., Ferro, M., Teste, M.-A., Geiselmann, J., & de Jong, H. (2021). Multiomics Study of Bacterial Growth Arrest in a Synthetic Biology Application. ACS Synthetic Biology, 10(11), 2910–2926. https://doi.org/10.1021/acssynbio.1c00115
Bouroumeau, A., Bussot, L., Hamaidia, S., Garcìa-Sandoval, A., Bergan-Dahl, A., Betton-Fraisse, P., Duley, S., Fournier, C., Aucagne, R., Adrait, A., Couté, Y., McLeer, A., Col, E., David-Boudet, L., Raskovalova, T., Jacob, M.-C., Vettier, C., Chevalier, S., Carras, S., … Emadali, A. (2021). CYCLON and NPM1 Cooperate within an Oncogenic Network Predictive of R-CHOP Response in DLBCL. Cancers, 13(23), 5900. https://doi.org/10.3390/cancers13235900
Clement, K., Reynaud, A., Defoort, M., Vysotskyi, B., Fortin, T., Lai, S.-H., Çumaku, V., Dominguez-Medina, S., Hentz, S., & Masselon, C. (2021). Requirements and attributes of nano-resonator mass spectrometry for the analysis of intact viral particles. Analytical and Bioanalytical Chemistry, 413(29), 7147–7156. https://doi.org/10.1007/s00216-021-03511-4
Imbert, A., Rompais, M., Selloum, M., Castelli, F., Mouton-Barbosa, E., Brandolini-Bunlon, M., Chu-Van, E., Joly, C., Hirschler, A., Roger, P., Burger, T., Leblanc, S., Sorg, T., Ouzia, S., Vandenbrouck, Y., Médigue, C., Junot, C., Ferro, M., Pujos-Guillot, E., … Thévenot, E. A. (2021). ProMetIS, deep phenotyping of mouse models by combined proteomics and metabolomics analysis. Scientific Data, 8(1), 311. https://doi.org/10.1038/s41597-021-01095-3
Boulan, B., Ravanello, C., Peyrel, A., Bosc, C., Delphin, C., Appaix, F., Denarier, E., Kraut, A., Jaquier-Sarlin, M., Fournier, A., Andrieux, A., Gory-Fauré, S., & Deloulme, J.-C. (2021). CRMP4-mediated fornix development involves semaphorin-3E signaling pathway. ELife, 10, e70361. https://doi.org/10.7554/eLife.70361
Deutsch, E. W., Omenn, G. S., Sun, Z., Maes, M., Pernemalm, M., Palaniappan, K. K., Letunica, N., Vandenbrouck, Y., Brun, V., Tao, S., Yu, X., Geyer, P. E., Ignjatovic, V., Moritz, R. L., & Schwenk, J. M. (2021). Advances and Utility of the Human Plasma Proteome. Journal of Proteome Research, 20(12), 5241–5263. https://doi.org/10.1021/acs.jproteome.1c00657