Energy and microalgae
Coordinator: Gilles Peltier (iBEB/SBVME/LB3M, UMR6191, CEA/Cadarache)
EDyP correspondent: Myriam Ferro
Funded by: ANR Bioenergy, 2008-2012 (French National Research Agency)
Chlamydomonas reinhardtii is a unicellular photosynthetic alga which has developed a metabolic capacity to produce hydrogen or accumulate lipid stores using water, CO2, and solar energy as its main resources. In some unfavourable situations (e.g. starvation), Chlamydomonas adapts its cellular metabolism to accumulate energy stores (starch, lipids). As part of the ALGOMICS project, EDyP is undertaking the proteomics part of a multidisciplinary project combining a wide range of “omics” approaches: proteomics, transcriptomics, genomics, metabolomics, and fluxomics. ALGOMICS aims to develop a “Systems biology” strategy to characterise Chlamydomonas mutants defective for reserve metabolism. The aim is to determine the points of control and regulation for algal metabolism http://www-heliobiotec.cea.fr/algomics.html.
For ALGOMICS, EDyP performed quantitative proteomics experiments on metabolically labelled Chlamydomonas cells (15N). In collaboration with the Cellular and Plant Physiology laboratory http://www-dsv.cea.fr/dsv/instituts/institut-de-recherches-en-technologies-et-sciences-pour-le-vivant-irtsv/unites/physiologie-cellulaire-vegetale-pcv/bienvenue, and Michael Hippler’s laboratory http://www.uni-muenster.de/Biologie.IBBP/aghippler/index.html, a sub-project is underway to develop a software tool predicting subcellular protein localisation specifically in algae. The tool, PredAlgo, recognises “signal” pre-sequences found at the N-terminal extremity of proteins. Development of PredAlgo is possible thanks to the use of proteomics analysis methods specifically targeting N-terminal peptides from Chlamydomonas proteins.