Dynamics of the chloroplast proteome

 As part of the ANR Glyco-Chloroplast project (Genoplante; 2007-2009), in collaboration with Norbert Rolland’s team, we established a database for the Arabidopsis thaliana chloroplast, the AT_Chloro database. The database listed over 1300 proteins found in the chloroplast or associated with it. Sub-chloroplast localisation information is available for all these proteins. The database was made available on a specially designed website http://www.grenoble.prabi.fr/at_chloro/  (Ferro et al., Molecular and Cellular Proteomics, 2010).

Among the envelope components of the chloroplast, the protein ceQORH has the specificity of containing a central transit sequence which is not cleaved after the protein has been imported into the chloroplast. This is in contrast with the majority of chloroplast proteins which lose their transit peptides upon import (Miras et al., 2002 http://www.ncbi.nlm.nih.gov/pubmed/12368288, 2007 http://www.ncbi.nlm.nih.gov/pubmed/17636260; patents FR0207729 and WO2004/001050 A1). Study of ceQORH revealed a previously unknown mechanism controlling protein trafficking between the cytosol and the chloroplast.
Chloroplast proteome dynamics is currently studied through two ANR projects and the GRAL project http://media.enseignementsup-recherche.gouv.fr/file/Fiches_Labex/86/1/GRAL_171861.pdf. Label-free quantitative proteomics approaches, particularly the AMT method, are used.

 

Chloro-Pro Project: Regulation of chloroplast proteome dynamics

Coordinator: Norbert Rolland (iRTSV/PCV, UMR5168, CEA/Grenoble)

EDyP correspondent: Myriam Ferro

Funded by: ANR Blanc, 2010-2014 (French National Research Agency)

The Chloro-Pro project aims to: i) Identify the chloroplast proteins regulated by an import mechanism similar to that used by ceQORH, ii) Identify signals controlling protein trafficking through the chloroplast envelope, and iii) Use quantitative proteomics to determine how deregulation of this mechanism affects chloroplast physiology.

 

Chloro-Types Project: Chloroplast adaptation to abiotic stress

Coordinator: Norbert Rolland (iRTSV/PCV, UMR5168, CEA/Grenoble)

EDyP correspondent: Myriam Ferro

Funded by: ANR genomics and plant biotechnology call, 2011-2014 (French National Research Agency)

The Chloro-types project aims to: i) Identify abiotic stressors affecting protein targeting to the chloroplast, ii) Determine the regulatory mechanisms induced by abiotic stress and controlling sub-cellular localisation of some proteins, and iii) Understand how regulatory mechanisms, induced by stress, affect chloroplast physiology.